Latest Update: 2014-04-07 13:16:20

蛋白質功能實驗室 Laboratory of Protein Function and Structure

研究領域 | Research Background

Protein Neuroscience, Enzymology, Protein Chemistry.

My research is concerned with the study of proteins, especially those involved in neuronal functions. The research involved isolation of proteins, determination of their structures and properties, cloning, and expression. The research is basic in nature, but significant relationships between this research and human disease. The research projects include two parts. First is focused on the interactions between the protein phosphatase-1 (PP1) and its regulatory proteins, which play a key role in dopaminergic signal transduction of the brain. My laboratory has identified the mechanism of PP1 regulated by one of its inhibitors, inhibitor-2. Recent studies have led to the elucidation of the first secondary structure of inhibitor-2 by NMR. Our final goals are to better understand the molecular mechanism in regulation of PP1, which may result in the design of drugs capable of mimicking or antagonizing the action of PP1, and the role of PP1 in regulation of neuronal functions. Purification and characterization of the novel PP1-binding proteins will be also included. A second area of research created recently concerns the mechanism in prevention of Ab polymerization and the structural differences of apoE isoforms, both of which are highly associated with the pathogenesis of the Alzheimer's disease (AD). Recently, we have planed to design the photo-sensitive peptides that can block the Ab polymerization. Our aim is to use NMR to elucidate 3-dimensional structure of Ab:peptide complex, providing the targets for design of therapeutic drugs in preventing the development of AD. Three common alleles of human apoE, e2, e3 and e4, are found, differing by a single amino acid substitution in residue-112 and –158 and showing the remarkable differences in their biochemistry, and cellular metabolism and biological functions. In this project, we focus on the structural studies of apoE isoforms by using NMR (collaborated with Dr. Ta-Hsien Lin, at VGH, Taipei). Our goals are to understand the relationship between protein structure and function.

實驗室成員

博士生

林郁姍、王梓帆、游惠君、黃光永、許瑞美、余芝嘉、劉韋亭

碩士生

張亦雯、范盛忠、吳宗憲、黃惠聖、呂洸彥、游智群、黃智源

專題生

任楚瑜、王涵羽、黃馨賢

已畢業學生

博士:紀孟君、張基煌、陳軍宇

碩士:

(100級)李允中、楊嘉倩、林亭妤、李思錦

(99級)張翔宇、張裕婷、周昱辰、洪詩惠

(98級)陳宜鈞、洪瑞霞、鄭雅鈴、沈俞伶

(97級)陳文斌、林秀蓉、黃光永、洪雅琳、蔡宜君、曾國枝

(96級)龔大翔

(95級)王琇嵐、蔡淑芬、顏嘉誼、李文星、郭政佑

(94級)張翰珍、蘇柔蓁

(93級)吳函蒼、洪瑋筌、林焜隆

(92級)陳軍宇、陳宏美、劉振鴻、黃韻如、陳逸軒、洪茂雄

(91級)王梓帆、沈政瑩、秦美玲、蘇家莉、王慧玲、謝富凱

(90級)蘇鈴珠、何詠寧、周秉杰、王嵐欣、蔡百騏、鄭皓軒