聯絡資訊 | Contact

Address	168, University Road, Min-Hsiung, Chia-Yi Taiwan, R. O. C.
    Tel	(05)2720411 ext. Office 66506  Lab 61517
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  Email	

學歷 | Education

Ph.D. Dept. of Chemical Engineering, Univ. of Maryland-College Park

現職 | Current Title

103/02 ~Present 副教授 國立中正大學生命科學系暨分子生物研究所暨生物醫學研究所

95/08 ~ 103/01  助理教授 國立中正大學生命科學系暨分子生物研究所暨生物醫學研究所

96/01 ~ Present 副研究員 國家生技醫療產業策進會

專長 | Professions

Nano-biotechnology

Molecular diagnostics

Biochips

Bioinformatics

Systems biology

Genomics

研究領域 | Research Background

本實驗室著重於跨領域的研究,充分整合分子生物與生物技術、生物資訊與系統生物學、柰米生醫和基因體等方面的專長,深耕以下四大研究領域:

抗體基因體學: 使用新世代定序技術(next generation sequencing),決定建康人和特定病人周邊血中淋巴球的抗體可變區基因列序,鑒別出疾病所產生的特異性抗體和抗原,未來將可據此發展出檢測的生物標記、疫苗和藥物。目前已從肺結核病病人血液中建構出單鏈抗體庫,進行全抗體基因定序,篩選出一些肺結核病人特有的血液生物標記,正展開臨床驗證中。肺癌相關研究也即將開展。

利用核糖體體外展示技術篩選癌細胞表面特有抗原: 利用核糖體展示技術從癌症病人周邊血中構建癌症的全人源抗體庫,以及人工合成的隨機胜肽庫,篩選並鑑定出數個與肺癌細胞表面抗原特異性結合的抗體或胜肽,進而可據此發展癌症血液診斷、antibody/peptide drug conjugate標靶治療、以及癌症疫苗。

奈米生醫

(1) 發展奈米材料生物反應評估平台: 建立一個較為接近真實狀態的細胞生物反應評估平台-巨噬細胞和目標細胞共同培養系統,並以基因體學為基礎,利用基因晶片和多變量統計分析,調查各種廣泛應用的奈米材料在細胞和老鼠組織中基因表現頻譜的變化和生物反應分子機轉,從而鑑定出各類細胞生物反應和老鼠組織病變的基因標誌。再利用這些基因生物標誌預測待測奈米材料產生可能的生物反應機制,可提供奈米材料應用於奈米生醫的安全性評估。

(2) 奈米藥物傳輸系統: 發展生物可容性之奈米材料攜帶肺結核以及癌症藥物傳輸系統,藉由有效穿越和精準傳輸至目標細胞與組織,大幅減少藥物使用量,達到減輕副作用和增進療效的目的。

分子診斷技術: 已開發出可攜式現場即時暨螢光奈米檢測檢測儀器,可應用于禽流感及新型流感快速檢驗之用。此外,我們也開發出結核菌抗原晶片偵測血液樣本中之九個結核菌抗體,並利用線性判別分析解析計算出感染分數,藉此確認此人類個體為健康、開放性結核菌感染或潛伏性結核菌感染。上述二個技術已獲得中華民國專利。


全人源抗體基因庫建構


疾病血液生物標記篩選


核醣體展示技術


結核菌抗原晶片


可攜式螢光奈米檢測檢測儀

論文與著作 | Publications

  1. Shi SD, Hsueh PR, Yang PC, Chou CC*. Identification of serum biomarkers for diagnosis of active and latent tuberculosis using Mycobacterium tuberculosis antigen array. Submitted to Clinical Infectious Diseases, 2107. (SCI IF=8.216, R/C=22/275, INFECTIOUS DISEASES)
  2. Chou CC*, Chen W, Hung Y, Mou CY. Molecular elucidation of biological response to mesoporous silica nanoparticles in vitro and in vivo. ACS Applied Materials & Interfaces, 2017, 9 (27): 22235–51. (SCI IF=7.504, R/C=22/275, MATERIALS SCIENCE, MULTIDISCIPLINARY)
  3. Chou CC*. Identification of the pivotal role of glutamate in enhancing insect cell growth using factor analysis. Cytotechnology, 2014, 66(5): 853-60. (SCI IF=1.857, R/C=88/158, BIOTECHNOLOGY & APPLIED MICROBIOLOGY)
  4. Chen PH, Hsiao KM, Chou CC*. Molecular characterization of toxicity mechanism of single-walled carbon nanotubes. Biomaterials, 2013, 34: 5661-5669. (SCI IF=8.402, R/C=1/33, MATERIALS SCIENCE, BIOMATERIALS)
  5. Chou CC*. Identification of bioactive yeastolate fractions responsible for insect cell growth and polyhedra production. Journal of Biochemical Technology, 20134 (3): 611-615.
  6. Chou CC*, Huang YH. Nucleic acid sandwich hybridization assay with quantum dot-induced fluorescence resonance energy transfer for pathogen detection. 2012, Sensors, 2012, 12: 16660-16672. (SCI IF=2.677, R/C=10/58, INSTRUMENTS & INSTRUMENTATION)
  7. Hsieh WY, Chou CC, Ho CC, Yu SL, Chen HY, Chou HY, Chen JJ, Chen HW, Yang PC. Single-walled carbon nanotubes induce airway hyper-reactivity and parenchymal injury in mice. American Journal of Respiratory Cell and Molecular Biology, 2012, 46: 257-267. (SCI IF=4.100, R/C=13/59, RESPIRATORY SYSTEM).
  8. Pan HC, Lin YJ, Li MW, Chuang HN, Chou CC*. Aquatic toxicity assessment of single-walled carbon nanotubes using zebrafish embryos. 2011, Journal of Physics: Conference series, 2011, 304: 012026.
  9. Chou CC, Hsiao HY, Hong QS, Chen CH, Peng YW, Chen HW and Yang PC. Single-walled carbon nanotubes can induce pulmonary injury in mouse model. Nano Letters, 2008, 8: 437-45. (SCI IF=12.712, R/C=11/275, MATERIALS SCIENCE, MULTIDISCIPLINARY).
  10. Chou CC and Peck K. Design and fabrication of spotted long oligonucleotide microarrays for gene expression analysis in Chapter 10 of Methods in Molecular Biology – Microarrays: Synthesis methods 2007; 381: 213-25 (book chapter)
  11. Sher YP, Chou CC, Chou RH, Wu HM, Chang WSW, Chen CH, Yang PC, Wu CW, Yu CL, and Peck K. Human kallikrein 8 protease confers a favorable clinical outcome in non–small cell lung cancer by suppressing tumor cell invasiveness. Cancer Research 2006, 66: 11763-70. (IF=9.122, R/C=15/217, ONCOLOGY).
  12. Chen HW, Su SF, Chien CT, Lin WH, Yu SL, Chou CC, Chen JW and Yang PC. Titanium dioxide nanoparticles induce emphysema-like lung injury in mice. FASEB Journal 2006, 20: E1732-412393-95. (IF=5.498, R/C=7/84, BIOLOGY, CELL BIOLOGY)
  13. Chou CC, Lee TT, Chen CH, Hsiao HY, Lin YL, Ho MH, Yang PC and Peck K. Design of microarray probes for virus identification and detection of emerging viruses at the genus level. BMC Bioinformatics 2006, 7: 232. (IF=2.448, R/C=10/57, MATHEMATICAL & COMPUTATIONAL BIOLOGY)
  14. Chen YA, Chou CC, Lu X, Slate EH, Peck K, Xu W, Voit EO and Almeida JS. A Multivariate prediction model for microarray cross-hybridization. BMC Bioinformatics 2006, 7: 101. (IF=2.448, R/C=10/57, MATHEMATICAL & COMPUTATIONAL BIOLOGY)
  15. Chou CC, Chen CH, Lee TT and Peck K. Optimization of probe length and the number of probes per gene for optimal microarray analysis of gene expression. Nucleic Acids Research 2004, 32:e99. (SCI IF=10.162, R/C=14/286, BIOCHEMISTRY & MOLECULAR BIOLOGY)

專利(含申請)

    1. 周正中、曾啟偉,「基於全體基因表現及生物反應路徑以及基因本體學之反應路徑分群方法」,中華民國專利證號I468968 (2015)。

    2. 周正中、曾啟偉,“Machine-implemented method for analyzing genome-wide gene expression profiling cross-reference to related application”,美國專利申請案號13/728291 (2012)。

    3. 周正中等,「判別開放性或潛伏性結核菌感染之方法」,中華民國專利證號I458978 (2014)。

    4. 范建得、羅仁權、吳明發、張益華、周正中、徐建業,「自搜尋擴充與分類修正之智慧財產權媒合系統與方法」,中華民國專利證號I346906 (2011)。

    5. 周正中等,「可溫控之微型生物分子反應晶片」,中華民國專利證號M373489 (2010)。

    6. Augustine Lin, PC Yang, CC Chou. Methods and applications of molecular beacon imaging for infectious disease and cancer detection. USPTO Application #: 20080032282, European Patent Application #: EP1979491, PCT Paten application #: PCT/US2006048853 (2006).

    7. Augustine Lin, PC Yang, CC Chou. 偵測傳染病及/或疾病標記之表現或突變之活體診斷及藥物基因體學之方法、分子標識探針及其診斷試劑套組. 中華民國專利申請案號TW095148836 (2006)。