聯絡資訊 | Contact

Address	168, University Road, Min-Hsiung Chia-Yi Taiwan, R. O. C.
    Tel	(05)2720411 ext. Office 53200  Lab 53201
    Fax	(05)2722871 
  Email	

學歷 | Education

1979-1984 B.S. Chemistry National Taiwan Normal University

1984-1986 M.S. Biochemistry National Taiwan University

1987-1992 phD. Biochemistry Cornell University Ph.D.

專長 | Professions

神經蛋白質、生物化學、酵素學

現職 | Current Title

Professor, Department of Life Science/Institute of Molecular Biology National Chung Cheng University

經歷 | Employment

Aug. 2003-2005 Chairman, Department of Life Science, National Chung Cheng University.

Aug. 2008-present Professor, Department of Life Science, National Chung Cheng University.

Aug. 2000-2008 Associate Professor, Institute of Molecular Biology, National Chung Cheng University.

Aug. 1995-July 2000 Associate Professor, Division of Biochemistry, Tzu Chi University.

Jan. 1992-July 1995 Postdoctor, Lab. of Molecular and Cellular Neuroscience, The Rockefeller University

(Advisor: Paul Greengard, The Noble Prize Laureate in 2000 for medicine and physiology)

研究領域 | Research Interests

Protein Neuroscience, Enzymology, Protein Chemistry

My research is concerned with the study of proteins, especially those involved in neuronal functions. The research involved isolation of proteins, determination of their structures and properties, cloning, and expression. The research is basic in nature, but significant relationships between this research and human disease. The research projects include two parts. First is focused on the interactions between the protein phosphatase-1 (PP1) and its regulatory proteins, which play a key role in dopaminergic signal transduction of the brain. My laboratory has identified the mechanism of PP1 regulated by one of its inhibitors, inhibitor-2. Recent studies have led to the elucidation of the first secondary structure of inhibitor-2 by NMR. Our final goals are to better understand the molecular mechanism in regulation of PP1, which may result in the design of drugs capable of mimicking or antagonizing the action of PP1, and the role of PP1 in regulation of neuronal functions. Purification and characterization of the novel PP1-binding proteins will be also included. A second area of research created recently concerns the mechanism in prevention of Ab polymerization and the structural differences of apoE isoforms, both of which are highly associated with the pathogenesis of the Alzheimer's disease (AD). Recently, we have planed to design the photo-sensitive peptides that can block the Ab polymerization. Our aim is to use NMR to elucidate 3-dimensional structure of Ab:peptide complex, providing the targets for design of therapeutic drugs in preventing the development of AD. Three common alleles of human apoE, e2, e3 and e4, are found, differing by a single amino acid substitution in residue-112 and –158 and showing the remarkable differences in their biochemistry, and cellular metabolism and biological functions. In this project, we focus on the structural studies of apoE isoforms by using NMR (collaborated with Dr. Ta-Hsien Lin, at VGH, Taipei). Our goals are to understand the relationship between protein structure and function.

特殊成就 | Major Awards and Honors

Research Award (甲等獎) (1997, 1999, National Science Council)

Outstanding Research Award (2005, National Chung Cheng University)

中正大學教師優良教學獎 (2007)

慈濟佛教大林分院顧問 (2007-present)

論文與著作

  1. Chuang TT, Ong PL, Wang TF, Huang HB, Chi MC, Lin LL (2012) Molecular characterization of a novel trehalose-6-phosphate hydrolase, TreA, from Bacillus licheniformis. Int. J. Biol. Macromol. 50, 459-470.
  2. Wang CC¶, Huang HB¶, Tsay HJ, Shiao MS, WinstonWu WJ, Chen YC*, Lin TH* (2012) Characterization of Aβ aggregation mechanism probed by congo red. J. Biomol. Struct. Dyn. 30, 160-169.
  3. Wang TF, Lai NS, Huang KY, Huang HL, Lu MC, Lin YS, Chen CY, Liu SQ, Lin TH*, Huang HB* (2012) Identification and characterization of the actin-binding motif of phostensin. Int. J. Mol. Sci. 13, 15967-15982.
  4. Lai NS, Yu CL, Yin WY, Yu HC, Huang HB, Tung CH, Lu MC. (2012) Combination of nifedipine and subtherapeutic dose of cyclosporin additivity suppresses mononuclear cells activation of patients with rheumatoid arthritis and normal individuals via Ca2+-calcineurin-nuclear factor of activated T cells pathway. Clin. Exp. Immunol. 168, 78-86.
  5. Liu MC, Lai NS, Chen HC, Yu HC, Huang KY, Tung CH. Huang HB, Yu CL. (2012) Decreased microRNA(miR)-145 and increased miR-224 expression in T cells from patients with systemic lupus erythematosus involved in lupus immunopathogenesis. Clin. Exp. Immunol. 171, 91-99.
  6. Ho TY, Huang KY, Huang TT, Huang YS, Ho HC, Chou P, Lin CH, Wei CK, Lian WC, Chen TC, Huang HB, Lee CC. (2012) The impact of influenza vaccinations on the adverse effects and hospitalization rate in the elderly: a national based study in an asian country. PloS One 7(11), e50337.
  7. Liu MC, Lai NS, Yin WY, Yu HC, Huang HB, Tung CH, Huang KY, Yu CL(2013). Anti-citrullinated protein antibodies activated ERK1/2 and JNK mitogen-activated protein kinases via binding to surface-expressed citrullinated GRP78 on mononuclear cells. J. Clin. Immunol. 33, 558-566.
  8. Lee MC, Lu MC, Lai NS, Liu SC, Yu HC, Lin TY, Hung SP, Huang HB, Yin WY (2013) Renal dysfunction by BK virus infection is correlated with activated T cell level in renal transplantation. J. Surg. Res. 180, 330-336.
  9. Chen YR, Huang HB, Lo CJ, Wang CC, Ho LK, Liu HT, Shiao MS, Lin TH*, Chen YC* (2013) Effect of Alanine Replacement of L17 and F19 on the aggregation and neurotoxicity of arctic-type A40. PLoS One 8(4), e61847.
  10. Yu HC, Lu MC, Li C, Huang HL, Huang KY, Liu SQ, Lai NS*, and Huang HB* (2013) Targeted delivery of an antigenic peptide to the endoplasmic reticulum: application for development of a peptide therapy for ankylosing spondylitis. PLoS One 8(10), e77451
  11. Tseng KC, Chou JL, Huang HB, Tseng CW, Wu SF, Chan MWY (2013) SOCS-1 Promoter Methylation and Treatment Response in Chronic Hepatitis C Patients Receiving Pegylated-Interferon/Ribavirin. J. Clin. Immunol. 33, 1110-1116.
  12. Lai NS, Yu HC, Chen HC, Yu CL, Huang HB, Lu MC (2013) Aberrant expression of microRNAs in T cells from patients with ankylosing spondylitis contributed to the immunopathogenesis. Clin. Exp. Immunol. 173, 47-57.
  13. Lin YS, Huang HL, Liu WT, Lin TH*, Huang HB* (2014) Identification of the high molecular weight isoform of phostensin. Int. J. Mol. Sci. 15, 1068-1079.
  14. Yu CC, Hung SK, Liao HF, Lee CC, Lin HY, Lai HC, Li SC, Ho HC, Huang HB*, Su YC* (2014) RAD001 Enhances the radiosensitivity of SCC4 oral cancer cells by inducing cell cycle arrest at the G2/M checkpoint. Anticancer Res. 34, 2927-2935.
  15. Huang SH, Ke SC, Lin TH, Huang HB, Chen YC (2014) Effect of C-terminal residues of A on copper binding affinity, structural conversion and aggregation. PLoS One 9(3), e90385.
  16. Lu MC, Yu CL, Chen HC, Yu HC, Huang HB, Lai NS (2015) Aberrant T cell expression of Ca2+ influx-regulated miRNAs in patients with systemic lupus erythematosus promotes lupus pathogenesis. Rheumatol. 54, 343-348.
  17. Lo CJ, Chyan CL, Chen YC, Chang CF, Huang HB*, Lin TH* (2015) Resonance assignments and secondary structure of apolipoprotein E C-terminal domain in DHPC micelles. Biomol. NMR Assign. 9, 187-190.
  18. Lo CJ, Wang CC, Huang HB, Chang CF, Shiao MS, Chen YC, Lin TH (2015) The Arctic mutation accelerates A aggregation in SDS through reducing the helical propensity of residues 15–25. Amyloid 22, 8-18.
  19. Yu HC, Huang KY, Lu MC, Huang HL, Liu WT, Lee WC, Liu SQ, Huang HB*, Lai NS* (2015) Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain. Int. J. Mol. Sci. 16, 8142-8150.
  20. Lai NS, Yu HC, Yu CL, Koo M, Huang HB, Lu MC* (2015) Anti-citrullinated protein antibodies suppress let-7a expression in monocytes from patients with rheumatoid arthritis and facilitate the inflammatory responses in rheumatoid arthritis. Immunobiology 220, 1351–1358.
  21. Yu HC, Lu MC, Huang KY, Huang HL, Liu SQ, Huang HB*, Lai NS* (2016) Sulfasalazine treatment suppresses the formation of HLA-B27 heavy chain homodimer in patients with ankylosing spondylitis. Int. J. Mol. Sci. 17, 46.
  22. Lu MC, Yu CL, Yu HC, Huang HB, Koo M, Lai NS* (2016) Anti-citrullinated protein antibodies promote apoptosis of mature human Saos-2 osteoblasts via cell-surface binding to citrullinated heat shock protein 60. Immunobiology 221, 76–83.
  23. Yu CC, Huang HB, Hung SK, Liao HF, Lee CC, Lin HY, Li SC, Ho HC, Hung CL, Su YC (2016) AZD2014 radiosensitizes oral squamous cell carcinoma by inhibiting AKT/mTOR axis and inducing G1/G2/M cell cycle arrest. PLoS ONE 11(3):e0151942.
  24. Liang CT1, Huang HB1, Wang CC, Chen YR, Chang CF, Shiao MS, Chen YC, Lin TH (2016) L17A/F19A substitutions augment the α-helicity of β-amyloid peptide discordant segment. PLoS ONE 11:e0154327. Lu MC, Yu HC, Yu CL, Huang, HB, Koo M, Tung CH, Lai NS(2016)Increased expression of long noncoding RNAs LOC100652951 and LOC100506036 in T cells from patients with rheumatoid arthritis facilitates the inflammatory responses. Immunol. Res.64:576-583.
  25. Yin, WY, Lee MC, Huang HB, Lu MC (2016) Increased gene expression of TGF-B in peripheral blood mononuclear cells from renal transplant patients with polyomavirus BK viremia. Clin. Transplant. 30: 393-398.
  26. Yu HC, Lai PH, Lai NS, Huang HB, Koo M, Lu MC (2016) Increased Serum Levels of Anti-Carbamylated 78-kDa Glucose-Regulated Protein Antibody in Patients with Rheumatoid Arthritis. Int. J. Mol. Sci.17: 1510.
  27. Liu WT, Huang KY, Lu MC, Huang HL, Chen CY, Cheng YL, Yu HC, Liu SQ, Lai NS*, Huang HB* (2017) TGF-B up-regulates the translation of USP15 via the PI3K/AKT pathway to promote p53 stability. Oncogene 36: 2715-2723.
  28. Yu HC, Huang KY, Liu MC, Huang HL, Liu SQ, Lai NS,*, Huang HB* (2017) Targeted delivery of the HLA-B*27-binding peptide into the endoplasmic reticulum suppresses the IL-23/IL-17 axis of immune cells in spondylarthritis. Mediat. Inflamm. (In press).

Conference papers

The 7th Symposium on Recent Advances in Biophysics (2001)

1.The Backbone Resonace Assignments of Inhibitor 1 - a Protein Inhibitor of Protein Phosphatase-1. Tzu-Chun Tang, Hsin-Tzu Liu, Hsien-Bin Huang, Yi-Chen Chen, Chia-Lin Chyan and Ta-Hsien Lin

2.The conformation Conversion of Gramicidin A from Double Helix to Helical Dimer in Phospholipids bilayer by Fluorescence Spectroscopy Spectroscopy. Ta-Hsien Lin, Hsien-Bin Huang, H. -A. Wei, S. -H. Shiao, B.A. Wallace, Y.C. Chen

The 10th Symposium on Recent Advances in Biophysics (2005)

4.NMR Study of the Thr35-phosphorylated Inhibitor-1. Yi-Choang Huang, Tzu-Chen Tang, Yi-Chen Chen, Hsien-Bin Huang, Yu-Chie Chen and Ta-Hsien Lin

5.Unfolding Study of b-Amyloid Peptide by NMR Spectroscopy. Ying-En Chen, Yi-Ru Chen, Chi-Jen Lo, Yi-Chen Chen, Chia-li Su, Hun-Gen Chang, Hsien-Bin Huang and Ta-Hsien Lin

6.Structural determination of the C-terminal domain of apolipoprotein E in lipid environment by NMR spectroscopy. Chi-Jen Lo, Yi-Chen, Hui-Ling Wang, Han-Tsang Wu, Hung-Mei Chen, Hsien-Bin Huang and Ta-Hsien Lin

The 11th Symposium on Recent Advances in Biophysics (2006)

7.Inhibition of b-amyloid peptide aggregation by rational mutation. Ying-En Chen, Chi-Jen Lo, Chih Ching Wang, Yi-Chen Chen, Chia-li Su, Hun-Gen Chang, Hsien-Bin Huang and Ta-Hsien Lin

The 15th Joint Annual Conference of Biomedical Science (2000)

  • 8.Identification and Characterization of the Isoform of Human Inhibitor-1. P104 Liu HT, Tsai LH, Kuo HC, Chen JH, Huang HB
  • The18th Joint Annual Conference of Biomedical Science (2003)

  • 9.Biochemical Characterizaton of Human Inhibitor 1. P103 Su LJ, Wang TF, Liu HT, Lin TH and Huang HB
  • 10.Characterization of the interaction between protein phosphatase 1 and inhibitor-2 by structure-function analysis, peptide competition and plasmon resonance. Tsai PC, Jeng HH, Wang LH, Shie FK, Lin YH, Chen YC, Lin TH and Huang HB
  • The 22th Joint Annual Conference of Biomedical Science (2007)

  • 11.Identification and characterization of phostensin, a novel protein phosphatase 1 F-actin cytoskeleton targeting subunit encoded by KIAA1949. P597 Kao SC, Chen CY, Wang SL, Hung WC and Huang HB
  • 12.Tzu-Fan Wang(王梓帆), Shu-Chen Kao2, Shiu-Lan Wang(王琇嵐), Chun-Yu Chen1(陳軍宇), Ning-Sheng Lai3, Hsien-Bin Huang(2009) .Identification and characterization of phostensin, a protein phosphatase 1 F-actin cytoskeleton targeting subunit. American Society for Biochemistry and Molecular Biology, Experimental Biology 2009, New Orleans, LA. (09-AB-2401-ASBMB, poster number C284).

    13.Chun-Yu Chen(陳軍宇), Ning-Sheng Lai, Jaw-Ji Yang, Hsien-bin Huang (2009) Identification and characterization of HePP1, a Heart protein phosphatase 1 binding protein, American Society for Biochemistry and Molecular Biology, Experimental Biology 2009, New Orleans, LA. (09-AB-2419-ASBMB, poster number C285)

    14.Yu shan Lin (林郁姍), Tzu-Fan Wang (王梓帆), Hui chun Yu (游惠君), Ruei shia Hung(洪瑞霞), Kuang yung Huang (黃光永), Ning sheng Lai, Hsien bin Huang. Immunohistochemical and immunocytochemical localization of human phostensin. Anaheim, California. April 24-28 2010 (poster)

    15.Hui-chunYu(游惠君), Ming-chi Lu, Ning-sheng Lai, Hsien-Bin Huang. Evaluate the B27-heavy chain dimer in patients with ankylosing spondylitis of Taiwan(台灣僵直性脊椎炎病人B27-heavy chain dimer 之評估) 14TH INTERNATIONAL CONGRESS OF IMMUNOLOGY(第十四屆國際免疫學學術研討會)Japan , Kobe(日本, 神戶) 2010.08.22~08.27

    16.Y.C. Zhou, T.F. Wang, C.Y. Chen, H.B. Huang*, Expression of the Recombinant Laccase in Aspergillus Oryzae, Asian Congress on Biotechnology 2011, May 11-15, 2011 in Shanghai , China

    17.Tzu-Fan Wang(王梓帆), Ruei-Shai Hung(洪瑞霞), Yu-Shan Lin(林郁姍), Ning-Sheng Lai, Ta-Hsien Lin, Hsien-Bin Huang. (2011) Structural and functional charctional characterization of the biochemical activity of phostensin. IX European symposium of the Protein Society. 瑞典斯德哥爾摩大學

    Research Funding:

    99-102計畫名稱:Phostensin之生化與細胞功能特性分析
     計畫編號:99-2320-B-194-003-MY3
     計畫金額:3,000,000

    96-98 計畫名稱:生化與功能性分析Phostensin特性, 一個編碼在KIAA1949並把1-型蛋白質磷酸酯酶標的到F-肌動蛋白之蛋白質
     計畫編號:96-2320-B-194-005-MY3
     計畫金額:5,310,000

    95計畫名稱:新型蛋白質1型磷酸水解酶調控蛋白, H3P和PP1RL, 的生化與功能特性分析
     計畫編號:95-2320-B-194-005
     計畫金額: 1,020,000

    94計畫名稱:鑑別與功能特性分析新的蛋白質, H3P (心臟特有之1型蛋白質磷酸水解酵素結合蛋白),一有潛力成為心肌損傷的鑑識標記蛋白
     計畫編號:94-2320-B-194-005-
     計畫金額:1,300,000

    93計畫名稱:1-型蛋白質磷酸水解脢與其抑制蛋白間之交互作用(3/3)
     計畫編號:93-2320-B-194-005
     計畫金額:1,697,400

    92計畫名稱:1-型蛋白質磷酸水解脢與其抑制蛋白間之交互作用(2/3)
     計畫編號:92-2320-B-194-005
     計畫金額:1,642,800

    91計畫名稱:1-型蛋白質磷酸水解脢與其抑制蛋白間之交互作用(1/3)
     計畫編號:91-2320-B-194-001
     計畫金額:1,858,800

    90計畫名稱:PP-1與其抑制蛋白間之分子結構和生化特性分析並純化腦神經細胞中新的PP-1結合蛋白(3/3)
     計畫編號:90-2320-B-194-001-
     計畫金額:1,157,200

    89計畫名稱:PP-1與其抑制蛋白間之分子結構和生化特性分析並純化腦神經細胞中新的PP-1結合蛋白(2/3)
     計畫編號:89-2320-B-194-004-
     計畫金額:1,158,400

    89計畫名稱:探討(A)1型-蛋白質磷酸水解抑制蛋白,DARPP-32和INHIBITOR(B)人類酯蛋白元E及B-
     計畫編號:89-2320-B-194-003-
     計畫金額:798,100

    89計畫名稱:PP-1與其抑制蛋白間之分子結構和生化特性分析並純化腦神經細胞中新的PP-1結合蛋白(1/3)
     計畫編號:89-2320-B-320-006-
     計畫金額:1,167,409

    88計畫名稱:腦部PROTEIN PHOSPHATASE-1結合蛋白之純化與特性分析並建立ATP-MG DEPENDENT PHOSPHATASE活性的調控機制
     計畫編號:88-2314-B-320-012-
     計畫金額:703,800

    87計畫名稱:PROTEIN PHOSPHATASE 1 與其抑制蛋白, DARPP-32 AND I-2, 間之交互作用與結構研究
     計畫編號:87-2732-B-320-001-
     計畫金額:604,200

    86計畫名稱:PROTEIN PHOSPHATASE 1 和 DARPP-32 間之交互作用與結構的研究
     計畫編號:86-2314-B-320-002-
     計畫金額:798,000