聯絡資訊 | Contact

Address	168, University Road, Min-Hsiung Chia-Yi Taiwan, R. O. C.
    Tel	(05)2720411 ext. Office 53200  Lab 53201
    Fax	(05)2722871 
  Email	

學歷 | Education

1979-1984 B.S. Chemistry National Taiwan Normal University

1984-1986 M.S. Biochemistry National Taiwan University

1987-1992 phD. Biochemistry Cornell University Ph.D.

專長 | Professions

神經蛋白質、生物化學、酵素學

現職 | Current Title

Professor, Department of Life Science/Institute of Molecular Biology National Chung Cheng University

經歷 | Employment

Aug. 2003-2005 Chairman, Department of Life Science, National Chung Cheng University.

Aug. 2008-present Professor, Department of Life Science, National Chung Cheng University.

Aug. 2000-2008 Associate Professor, Institute of Molecular Biology, National Chung Cheng University.

Aug. 1995-July 2000 Associate Professor, Division of Biochemistry, Tzu Chi University.

Jan. 1992-July 1995 Postdoctor, Lab. of Molecular and Cellular Neuroscience, The Rockefeller University

(Advisor: Paul Greengard, The Noble Prize Laureate in 2000 for medicine and physiology)

研究領域 | Research Interests

Protein Neuroscience, Enzymology, Protein Chemistry

My research is concerned with the study of proteins, especially those involved in neuronal functions. The research involved isolation of proteins, determination of their structures and properties, cloning, and expression. The research is basic in nature, but significant relationships between this research and human disease. The research projects include two parts. First is focused on the interactions between the protein phosphatase-1 (PP1) and its regulatory proteins, which play a key role in dopaminergic signal transduction of the brain. My laboratory has identified the mechanism of PP1 regulated by one of its inhibitors, inhibitor-2. Recent studies have led to the elucidation of the first secondary structure of inhibitor-2 by NMR. Our final goals are to better understand the molecular mechanism in regulation of PP1, which may result in the design of drugs capable of mimicking or antagonizing the action of PP1, and the role of PP1 in regulation of neuronal functions. Purification and characterization of the novel PP1-binding proteins will be also included. A second area of research created recently concerns the mechanism in prevention of Ab polymerization and the structural differences of apoE isoforms, both of which are highly associated with the pathogenesis of the Alzheimer's disease (AD). Recently, we have planed to design the photo-sensitive peptides that can block the Ab polymerization. Our aim is to use NMR to elucidate 3-dimensional structure of Ab:peptide complex, providing the targets for design of therapeutic drugs in preventing the development of AD. Three common alleles of human apoE, e2, e3 and e4, are found, differing by a single amino acid substitution in residue-112 and –158 and showing the remarkable differences in their biochemistry, and cellular metabolism and biological functions. In this project, we focus on the structural studies of apoE isoforms by using NMR (collaborated with Dr. Ta-Hsien Lin, at VGH, Taipei). Our goals are to understand the relationship between protein structure and function.

特殊成就 | Major Awards and Honors

Research Award (甲等獎) (1997, 1999, National Science Council)

Outstanding Research Award (2005, National Chung Cheng University)

中正大學教師優良教學獎 (2007)

慈濟佛教大林分院顧問 (2007-present)

論文與著作

  1. Chu, S.T., Yu, C.Y., Huang, H.B. and Chen, Y.H. (1990) Heterogeneous forms of glucose-6-phosphate dehydrogenase in human erythrocytes. J. Chinese Biochem. Soc. 18, No. 1, 1-9.
  2. Rose, J., Breslow, E., Huang, H.B. and Wang, B.C. (1991) Crystallographic analysis of the neurophysin-oxytocin complex. J. Mol. Biol. 221, 43-45.
  3. Huang, H.B. and Breslow, E. (1992) Identification of the unstable neurophysin disulfide and location to the hormone-binding site: relationship to folding-unfolding pathway. J. Biol. Chem. 267, 6750-6756.
  4. Breslow, E., Mishra, P.K., Huang, H.B. and Bothner-by, A. (1992) Interchanging conformers of bovine neurophysin I in the unliganded dimer state. Biochemistry 31, 11397-11404.
  5. Huang, H.B., Laborde, T. and Breslow, E. (1993) Modulation of allosteric interactions in neurophysin induced by succinylation of ser-56 or cleavage of residues. Biochemistry 31, 10743-10749.
  6. Huang, H.B., Wellner, D., Naude, R., Oelofsen, W., Oosthuizen, M.M.J. and Breslow, E. (1994) Amino acid sequence and properties of vasopressin-associated elephant neurophysin. Int. J. Peptide Protein Res. 44, 270-277.
  7. Desdouits, F., Cheetham, J.J., Huang, H.B., Kwon, Y.G., da Cruz e Silva, E.F., Denefle, P., Ehrlich, M.E., Nairn, A.C., Greengard, P. and Girault, J.A. (1995) Mechanism of inhibition of protein phosphatase 1 by DARPP-32: studies with recombinant DARPP-32 and Synthetic peptides. Biochem. Biophys. Res. Commun. 206, 652-658.
  8. Goldberg, J., Huang, H.B., Kwon, Y.G., Greengard, P., Nairn, A.C. and Kuriyan, J. (1995) Three-dimensional structure of the catalytic subunit of protein serine/threonine phosphatase-1. Nature 376, 745-753.
  9. Huang, H.B., Horiuchi, A., Goldberg, J., Greengard, P. and Nairn, A.C. (1997) Site-directed mutagenesis of amino acid residues of protein phosphatase-1 involved in catalysis and inhibitor binding. Proc. Natl. Acad. Sci. USA 94, 3540-3535.
  10. Kwon, Y.G., Huang, H.B., Desdouits, F., Girault, J.A., Greengard, P. and Nairn, A.C. (1997) Characterization of the interaction between protein phosphatase-1 and DARPP-32: DARPP-32 peptide antagonize the interaction of protein phosphatase-1 with binding proteins. Proc. Natl. Acad. Sci. USA 94, 3536-3541.
  11. Lin S.R., Huang, H.B., Wu, B.N. and Chang, L.S. (1998) Characterization and cloning of long neurotoxin homolog from naja naja atra. Biochem. Mol. Biol. Int. 46, 1211-1217.
  12. Aggen, J.B., Humphrey, J.M., Gauss, C.M., Huang, H.B., Nairn, A.C. and Chamberlin, R. (1999) The design, synthesis, and biolobical evaluation of analogs of the serine-threonine protein phosphatase 1 and 2A selective inhibitor microcystin LA: rational modifications imparting PP-1 selectivity. Bioorg. Med. Chem 7, 543-564.
  13. Huang, H.B.*, Horiuchi, A., Watanabe, T., Shih, S.R., Tsay, H.J., Li., H.C., Greengard, P. and Nairn, A.C*. (1999) Characterization of the inhibition of protein phosphatase-1 by DARPP-32 and inhibitor-2. J. Biol. Chem 274, 7870-7878.
  14. Chang,L.S., Lin, S.K., Huang, H.B. and Hsiao, H.S.M. (1999) Genetic organization of alpha-bungarotoxin from Bungarus multicinctus (Taiwan banded krait): Evidence showing the production of alpha-bungarotoxin isotoxins is not derived from edited mRNA. Nucleic Acids Research 27, 3970-3975.
  15. Chang, L.S., Huang, H.B., and Lin, S.R. (2000) The multiplicity of cardiotoxins from Naja naja atra (Taiwan cobra) venom. Toxicon 38, 1065-1076.
  16. Connor, J.H., Frederick, D., Huang, H.B., Yang, J., Helps, N.R., DePaoli-Roach, A., Cohen, P.T.W., Tatchell, K., Nairn, A.C. and Shenolikar, S. (2000) Cellular Mechanism Regulating Protein Phosphatase-1: A key Functional Interaction between Inhibitor-2 and the PP1 Catalytic Subunit. J. Biol. Chem. 275, 18670-18675.
  17. Chang, L.S., Lin, S.R., Hu, W.P., Wu, B.N., and Huang, H.B. (2000) Structure-function studies on Taiwan cobra long neurotoxin homolog. Biochem. Biophys. Acta 1480, 293-301.
  18. Huang, H.B., Chen, Yi-chen, Tsai, Li-huang, Wang, Hui-chun, Lin, Fang-min, Shiao, Ming-shi, and Lin, Ta-hsien Lin (2000) Expression and assignment of the 1H, 15N, and 13C resonances of inhibitor-2, the inhibitor of protein phosphatase-1. J. Biomol. NMR 17, 359-360.
  19. Watanabe, T., Huang, H.B., Horiuchi, A., Hsieh-Wilson, L., Allen, P.B., Shenolikar, S., Greengard, P. and Nairn, A.C. (2001) Protein phosphatase 1 regulation by inhibitors and targeting subunits. Proc. Natl. Acad. Sci. 98, 3080-3085.
  20. Huang, H.B., Chen, Y.C., Horiuchi, A., Tsai, L.H., Liu, H.T., Chyan, C.L., Lin, F.M., Greengard, P., Nairn, A.C., Hsieh, M.J., Liu, C.K., Shiao, M.S., and Lin, T.H. (2001) Backbone 1H, 15N, and 13C resonance assignments of ARPP-19. J. Biomol. NMR 19, 383-384.
  21. Chang, L.S., Chung, C., Huang, H.B., Lin, S.R. (2001) Purification and characterization of a chymotrypsin inhibitor from the venom of Ophiophagus hannah (king cobra). Biochem. Biophys. Res. Commun. 283, 862-867.
  22. Chyan, C.L., Tang, T.C., Chen, Y.C., Liu, H.T., Lin, F.M., Liu, C.K., Hsieh, M.J., Shiao, M.S., Huang, H.B., and Lin, T.H*. (2001) Backbone 1H, 15N, and 13C resonance assignments of inhibitor-1—a protein inhibitor of protein phosphatase-1. J. Biomol. NMR 21, 287-288.
  23. Gulledge, B., Aggen, J.B., Huang, H.B., Nairn, A.C. and Chamberlin, R. (2002) The microcystins and nodularins: cyclic polypeptide inhibitors of PP1 and PP2A. Current Medicinal Chemistry 9, 1903-1915.
  24. Hung, C.J., Huang, P.C., Lu, S.C., Li, Y.H., Huang, H.B., Lin, B.F., Chang, S.J., and Chou, H.F. (2002) Plasma homocysteine levels in Taiwanese vegetarians are higher than those of omnivores. J. Nutr. 132, 152-158.
  25. Liu, H.T., Lin, T.H., Kuo, H.C., Chen, Y.C., Tsay, H.J., Jeng, H.H., Tsai, P.C., Shie, F.C., Chen, J.H., and Huang, H.B.* (2002) Identification of the alternative splice products encoded by the human protein phosphatase inhibitor-1 gene. Biochem. Biophys. Res. Commun. 291, 1293-1296.
  26. Chang, L.S., Liou, J.C., Lin, S.R., and Huang, H.B. (2002) Purification and characterization of neurotoxin from the venom of Ophiophagus hannah (king cobra). Biochem. Biophys. Res. Commun. 294, 574-578.
  27. Liu, W., Colby, D.A., Sheppeck, J.E., Huang, H.B., Nairn, A.C., and Chamberlin, A.R. (2003) The selective inhibition of phosphatase by natural toxin: the anhydride domain of tautomycin is not a primary factor in controlling PP1/PP2A Sselectivity. Bioorg. Med. Chem. Lett. 13, 1597-1600.
  28. Colby, D.A., Liu, W., Sheppeck, J.E., Huang, H.B., Nairn, A.C., and Chamberlin, A.R. (2003) A new model of tautomycin-PP1 complex that is not analogous to the corresponding okadaic acid structure. Bioorg. Med. Chem. Lett. 13, 1601-1605.
  29. Sung, Y.J., Cheng, C.I., Chen, C.S., Huang, H.B., Wu, P.C., Shiao, M.S., and Tsay, H.J. (2003) Distinct mechanisms account for b-amyloid toxicity in PC12 and Differentiated PC12 neuronal cells. J. Biomed. Sci. 10, 379-388.
  30. Watanabe, T., da Cruz e Silva, E., Huang, H.B., Starkova, N., Kwon, Y.G., Horiuchi, A., Greengard, P., and Nairn, A.C. (2003) Preparation and characterization of recombinant protein phosphatase 1. Methods Enzymol. 366, 319-338.
  31. Lin, T.H., Chen, Y.C., Chyan, C.L., Tsay, L.H., Tang, T.C., Jeng, H.H., Lin, F.M., and Huang, H.B.* (2003) Phosphorylation of glycogen synthase kinase of inhibitor-2 does not change its structure in free state. FEBS Lett. 554, 253-256.
  32. Lin, T.H., Huang, J.W., Huang, H.B., Chen, Y.C., Liu, C.Y., Lo, C.J., Tang, T.C., Chyan, C.L. (2004) 1H, 15N, and 13C resonance assignments of complexed with the calmodulin-binding domain of protein phosphatase 2B. J. Biomol. NMR. 29, 531-532.
  33. Lin, T.H., Huang, Y.C., Chin, M.L., Chen, Y.C., Jeng, H.H., Lin, F.M., Shiao, M.S., Horiuchi, A., Greengard, P., Nairn, A.C., and Huang, H.B.* (2004) Backbone 1H, 15N, and 13C resonance assignments of DARPP-32 (dopamine and cAMP-regulated phosphoprotein, Mr. 32,000)—a protein inhibitor of protein phosphatase-1 J. Biomol. NMR. 28, 413-414.
  34. Lin, T.H., Huang, H.B., Wei, H.A., Shiao, S.H., Chen, Y.C. (2005) The effect of temperature and lipid on the conformational transition of gramicidin A in lipid vesicles. Biopolymers 78, 179-186.
  35. Huang HB, ChiMC, Hsu WH, Liang WC, Lin LL (2005) Construction and one-step purification of Bacillus kaustophilus leucine aminopeptidase fused to the starch-binding domain of Bacillus sp. Strain TS-23 a-amylase. Biopro. Biosyst. Eng. 27, 389-398.
  36. Chyan CL, Huang PC, Lin TH, Huang JW, Lin SS, Huang HB, Chen YC (2005) Purification, crystallization and preliminary crystallographic studies of a calmodulin-OLFp hybrid molecule. Acat. Cryst. F61, 785-787.
  37. Lin TH, Tsai PC, Liu HT, Chen YC, Wang LS, Shie FK, Huang HB* (2005) Characterization of the protein phosphatase 1-binding motifs of inhibitor-2 and DARPP-32 by surface plasmon resonance. J. Biochem. 138, 697 – 700.
  38. Chi MC, Huang HB, Liu JS, Wang WC, Liang WC, Lin LL (2006) Residues threonine 346 and leucine 352 are critical for the proper function of Bacillus kaustophilus leucine aminopeptidase. FEMS Microbiol. Lett. 260, 156-161.
  39. Chen YM, Lee TH, Lee SJ, Huang HB, Huang R, Chou HN (2006) Comparison of protein phosphatase inhibition activies and mouse toxicities of microcystins. Toxicon 47, 742-746.
  40. Chang LS, Lin SR, Huang HB (2006) Disulfide isomerization and thiol-disulfide exchange of long neurotoxins from the venom of Ophiophagus hannah. Arch. Biochem. Biophy. 454, 181-188.
  41. Chen YR, Huang HB, Chyan CL, Shiao MS, Lin TH, Chen YC (2006) The effect of Ab conformation on the metal affinity and aggregation mechanism studies by circular dichroism spectroscopy. J. Biochem. 139, 733-740.
  42. Chang LS, Chen KC, Lin SR, Huang HB (2006) Purification and characterization of Ophiophagus Hannah cytotoxin-like proteins. Toxicon 48, 429-436.
  43. Liao MQ, Tzeng YI, Chang LY, Huang HB, Lin TH, Chyan CL, Chen YC (2007) The correlation between neurotoxicity, aggregative ability and secondary structure studies by sequence truncated Ab peptides. FEBS Let. 581, 1161-1165.
  44. Huang HB*, Chen YC, Lee TT, Huang YC, Liu HT, Liu CK, Tsay HJ, Lin TH* (2007) Structural and biochemical characterization of inhibitor-1a. Proteins 68, 779-788.
  45. Kao SC, Chen CY, Wang SL, Yang JJ, Hung WC, Chen YC, Lai NS, Liu HZ, Huang HL, Chen HC, Lin TH*, Huang HB* (2007) Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit. Biochem. Biophys. Res. Commum. 356, 594-598.
  46. Chi MC, Liu JS, Wang WC, Lin LL*, Huang HB* (2007) Site-directed mutagenesis of the conserved Ala348 and Gly350 residues at the putative site of Bacillus kaustophilus leucine aminopeptidase. Biochimie 90, 811-819.
  47. Chi MC, Lu RQ, Lin LL*, Huang HB* (2007) Characterization of Bacillus kaustophilus leucine aminopeptidase immobilized in Ca-alginate/k-carrageenan beads. Biochem. Eng. J.39, 376-382.
  48. Chi MC, Ong PL, Hsu WE, Chen YH, Huang HB*, Lin LL* (2008) Role of the invariant Asn345 and Asn435 residues in a leucine aminopeptidase from Bacillus kaustophilus as evaluated by site-directed mutagenesis. Int. J. Biol. Macromol. 43, 481-487.
  49. Chang CH, Peng CH, Chen KC, Huang HB, Chiu WT, Peng RY (2009) Shock membrane electropotential drops and limited diffusive distance of b-amyloids in cerebral neurons are detrimental enhancement to Alzheimer’s diseases. Colloids and Surfaces B: Biointerfaces. 73, 339-345.
  50. Lai NS, Wang TF, Wang SL, Chen CY, Yen JY, Huang HL, Li C, Huang KY, Liu SQ, Lin TH*, Huang HB* (2009) Phostensin caps to the pointed end of actin filaments and modulates actin dynamics. Biochem. Biophys. Res. Commum. 387, 676-681.
  51. Huang YC, Chen YC, Tsay HJ, Chyan CL, Chen CY, Huang HB* and Lin TH* (2010) The effect of PKA-Phosphorylation on the structure of inhibitor-1 studied by NMR spectroscopy. J. Biochem. 147, 273-278.
  52. Chen CY, Lai NS, Yang JJ, Huang HL, Hung WC, Li C, Lin TH*, Huang HB* (2010) FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1). Biochem. Biophys. Res. Commum. 391, 698-702.
  53. Lu MC, Lai NS, Yu HC, Huang HB, Hsieh SC, Yu CL (2010) Anti-citrullinated protein antibodies bind surface-expressed citrullinated Grp78 on monocytes/macrophages and stimulate tumor necrosis factor α production. Arthritis Rheum. 62, 1213-1223.
  54. Chen YR1, Huang HB1, Lo CJ, Wang CC, Su CL, Liu HZ, Shiao MS, Lin TH*, Chen YC* (2011)Aβ40(L17A/F19A) mutant diminishes the aggregation and neurotoxicity of Aβ40. Biochem. Biophys. Res. Commum. 405, 91-95.
  55. Chen YC, Lee CC, Huang CY, Huang HB, Yu CC, Ho YC, Su YC (2011) Haptoglobin polymorphism as a risk factor for chronic kidney disease: A case-control study. Am. J. Nephrol. 33, 510-514.
  56. Lin YS1, Huang KY1, Wang TF, Huang HL, Yu HC, Yen JY, Hung SH, Liu SQ, Lai NS*, Huang HB* (2011) Immunolocalization of Phostensin in Lymphatic Cells and Tissues. J. Histochem. Cytochem. 59, 741-749.
  57. Wang CC¶, Huang HB¶, Tsay HJ, Shiao MS, WinstonWu WJ, Chen YC*, Lin TH* (2012) Characterization of Aβ aggregation mechanism probed by congo red. J. Biomol. Struct. Dyn. 30, 160-169.

Conference papers

The 7th Symposium on Recent Advances in Biophysics (2001)

1.The Backbone Resonace Assignments of Inhibitor 1 - a Protein Inhibitor of Protein Phosphatase-1. Tzu-Chun Tang, Hsin-Tzu Liu, Hsien-Bin Huang, Yi-Chen Chen, Chia-Lin Chyan and Ta-Hsien Lin

2.The conformation Conversion of Gramicidin A from Double Helix to Helical Dimer in Phospholipids bilayer by Fluorescence Spectroscopy Spectroscopy. Ta-Hsien Lin, Hsien-Bin Huang, H. -A. Wei, S. -H. Shiao, B.A. Wallace, Y.C. Chen

The 10th Symposium on Recent Advances in Biophysics (2005)

4.NMR Study of the Thr35-phosphorylated Inhibitor-1. Yi-Choang Huang, Tzu-Chen Tang, Yi-Chen Chen, Hsien-Bin Huang, Yu-Chie Chen and Ta-Hsien Lin

5.Unfolding Study of b-Amyloid Peptide by NMR Spectroscopy. Ying-En Chen, Yi-Ru Chen, Chi-Jen Lo, Yi-Chen Chen, Chia-li Su, Hun-Gen Chang, Hsien-Bin Huang and Ta-Hsien Lin

6.Structural determination of the C-terminal domain of apolipoprotein E in lipid environment by NMR spectroscopy. Chi-Jen Lo, Yi-Chen, Hui-Ling Wang, Han-Tsang Wu, Hung-Mei Chen, Hsien-Bin Huang and Ta-Hsien Lin

The 11th Symposium on Recent Advances in Biophysics (2006)

7.Inhibition of b-amyloid peptide aggregation by rational mutation. Ying-En Chen, Chi-Jen Lo, Chih Ching Wang, Yi-Chen Chen, Chia-li Su, Hun-Gen Chang, Hsien-Bin Huang and Ta-Hsien Lin

The 15th Joint Annual Conference of Biomedical Science (2000)

  • 8.Identification and Characterization of the Isoform of Human Inhibitor-1. P104 Liu HT, Tsai LH, Kuo HC, Chen JH, Huang HB
  • The18th Joint Annual Conference of Biomedical Science (2003)

  • 9.Biochemical Characterizaton of Human Inhibitor 1. P103 Su LJ, Wang TF, Liu HT, Lin TH and Huang HB
  • 10.Characterization of the interaction between protein phosphatase 1 and inhibitor-2 by structure-function analysis, peptide competition and plasmon resonance. Tsai PC, Jeng HH, Wang LH, Shie FK, Lin YH, Chen YC, Lin TH and Huang HB
  • The 22th Joint Annual Conference of Biomedical Science (2007)

  • 11.Identification and characterization of phostensin, a novel protein phosphatase 1 F-actin cytoskeleton targeting subunit encoded by KIAA1949. P597 Kao SC, Chen CY, Wang SL, Hung WC and Huang HB
  • 12.Tzu-Fan Wang(王梓帆), Shu-Chen Kao2, Shiu-Lan Wang(王琇嵐), Chun-Yu Chen1(陳軍宇), Ning-Sheng Lai3, Hsien-Bin Huang(2009) .Identification and characterization of phostensin, a protein phosphatase 1 F-actin cytoskeleton targeting subunit. American Society for Biochemistry and Molecular Biology, Experimental Biology 2009, New Orleans, LA. (09-AB-2401-ASBMB, poster number C284).

    13.Chun-Yu Chen(陳軍宇), Ning-Sheng Lai, Jaw-Ji Yang, Hsien-bin Huang (2009) Identification and characterization of HePP1, a Heart protein phosphatase 1 binding protein, American Society for Biochemistry and Molecular Biology, Experimental Biology 2009, New Orleans, LA. (09-AB-2419-ASBMB, poster number C285)

    14.Yu shan Lin (林郁姍), Tzu-Fan Wang (王梓帆), Hui chun Yu (游惠君), Ruei shia Hung(洪瑞霞), Kuang yung Huang (黃光永), Ning sheng Lai, Hsien bin Huang. Immunohistochemical and immunocytochemical localization of human phostensin. Anaheim, California. April 24-28 2010 (poster)

    15.Hui-chunYu(游惠君), Ming-chi Lu, Ning-sheng Lai, Hsien-Bin Huang. Evaluate the B27-heavy chain dimer in patients with ankylosing spondylitis of Taiwan(台灣僵直性脊椎炎病人B27-heavy chain dimer 之評估) 14TH INTERNATIONAL CONGRESS OF IMMUNOLOGY(第十四屆國際免疫學學術研討會)Japan , Kobe(日本, 神戶) 2010.08.22~08.27

    16.Y.C. Zhou, T.F. Wang, C.Y. Chen, H.B. Huang*, Expression of the Recombinant Laccase in Aspergillus Oryzae, Asian Congress on Biotechnology 2011, May 11-15, 2011 in Shanghai , China

    17.Tzu-Fan Wang(王梓帆), Ruei-Shai Hung(洪瑞霞), Yu-Shan Lin(林郁姍), Ning-Sheng Lai, Ta-Hsien Lin, Hsien-Bin Huang. (2011) Structural and functional charctional characterization of the biochemical activity of phostensin. IX European symposium of the Protein Society. 瑞典斯德哥爾摩大學

    Research Funding:

    99-102計畫名稱:Phostensin之生化與細胞功能特性分析
     計畫編號:99-2320-B-194-003-MY3
     計畫金額:3,000,000

    96-98 計畫名稱:生化與功能性分析Phostensin特性, 一個編碼在KIAA1949並把1-型蛋白質磷酸酯酶標的到F-肌動蛋白之蛋白質
     計畫編號:96-2320-B-194-005-MY3
     計畫金額:5,310,000

    95計畫名稱:新型蛋白質1型磷酸水解酶調控蛋白, H3P和PP1RL, 的生化與功能特性分析
     計畫編號:95-2320-B-194-005
     計畫金額: 1,020,000

    94計畫名稱:鑑別與功能特性分析新的蛋白質, H3P (心臟特有之1型蛋白質磷酸水解酵素結合蛋白),一有潛力成為心肌損傷的鑑識標記蛋白
     計畫編號:94-2320-B-194-005-
     計畫金額:1,300,000

    93計畫名稱:1-型蛋白質磷酸水解脢與其抑制蛋白間之交互作用(3/3)
     計畫編號:93-2320-B-194-005
     計畫金額:1,697,400

    92計畫名稱:1-型蛋白質磷酸水解脢與其抑制蛋白間之交互作用(2/3)
     計畫編號:92-2320-B-194-005
     計畫金額:1,642,800

    91計畫名稱:1-型蛋白質磷酸水解脢與其抑制蛋白間之交互作用(1/3)
     計畫編號:91-2320-B-194-001
     計畫金額:1,858,800

    90計畫名稱:PP-1與其抑制蛋白間之分子結構和生化特性分析並純化腦神經細胞中新的PP-1結合蛋白(3/3)
     計畫編號:90-2320-B-194-001-
     計畫金額:1,157,200

    89計畫名稱:PP-1與其抑制蛋白間之分子結構和生化特性分析並純化腦神經細胞中新的PP-1結合蛋白(2/3)
     計畫編號:89-2320-B-194-004-
     計畫金額:1,158,400

    89計畫名稱:探討(A)1型-蛋白質磷酸水解抑制蛋白,DARPP-32和INHIBITOR(B)人類酯蛋白元E及B-
     計畫編號:89-2320-B-194-003-
     計畫金額:798,100

    89計畫名稱:PP-1與其抑制蛋白間之分子結構和生化特性分析並純化腦神經細胞中新的PP-1結合蛋白(1/3)
     計畫編號:89-2320-B-320-006-
     計畫金額:1,167,409

    88計畫名稱:腦部PROTEIN PHOSPHATASE-1結合蛋白之純化與特性分析並建立ATP-MG DEPENDENT PHOSPHATASE活性的調控機制
     計畫編號:88-2314-B-320-012-
     計畫金額:703,800

    87計畫名稱:PROTEIN PHOSPHATASE 1 與其抑制蛋白, DARPP-32 AND I-2, 間之交互作用與結構研究
     計畫編號:87-2732-B-320-001-
     計畫金額:604,200

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     計畫金額:798,000