聯絡資訊 | Contact

Address	168, University Road, Min-Hsiung Chia-Yi Taiwan, R. O. C.
    Tel	(05)2720411 ext. Office 66501  Lab 61511
    Fax	(05)2722871 
  Email	

學歷 | Education

1973 - 1977 學士 高雄醫學院藥學系

1977 - 1979 碩士 國立台灣大學生化科學研究所

1983 - 1989 博士 美國密西根大學生物化學系

1977 B.S. in Pharmacy at KaohsiungMedicalCollege at Taiwan

1979 M.S. in Biochemical Sciences at NationalTaiwanUniversity

1989 Ph.D. in Biological Chemistry at University of Michigan

專長 | Professions

細胞生理學、受體生物學、生物科技

現職 | Current Title

Associate Professor, Department of Life Science/Institute of Molecular Biology National Chung Cheng University

經歷 | Experience

1989 - 1991 研究員 美國密西根大學生物化學系

1991 - 1997 資深研究員 美國密西根大學生理學系

1997 - 1998 資深博士後研究員 中央研究院生物化學研究所

1998 - 2004 副教授 臺北醫學大學細胞及分子生物研究所

2004 - 副教授 國立中正大學生命科學系暨分子生物研究所

特殊經歷 | Professional Experiences

1981-83 Assistant Researcher, Institute of Biological Chemistry, Academia Sinica, Taiwan

1989-91 Research Fellow, Department of Biological Chemistry, University of Michigan

1991-97 Sr. Research Fellow, Department of Physiology, University of Michigan

1997-98 Sr. Postdoctoral Fellow, Institute of Biological Chemistry, Academia Sinica

1998-04 Associate Professor, Graduate Institute of Cellular and Molecular Biology, TaipeiMedicalUniversity

Sequence Data on GeneBank

* Tseng, M.-J., Chang, P. & Tsai, C.-L. (2003) Bacillus firmus BH0898-like protein and xylanase 11A genes, complete cds. Accession number: AY376352.

* Tseng, M.-J., Chang, P. & Tsai, C.-L. (2003) Bacillus firmus BH2133-like protein gene, partial cds; and xylanase 10A and BH2119-like protein genes, complete cds. Accession number: AY376353.

研究領域 | Research Interests

Notch signaling system

Notch signaling system—This ongoing work focuses on resolving the activated Notch complex and elucidating the molecular mechanism(s) of Notch signaling pathway in cell-fate determination and tumorgenesis. Receptor biology--This work focused on the structure-function relationship of G-protein coupled receptors. PCR methodology was employed to construct the chimeric or mutated receptors and various stable cell lines expressing wild type or mutated receptors were obtained. In addition, the ligand-receptor interaction, second messengers (IP3, cAMP, arachidonate) production, and Ca2+ signaling were measured. We also studied the functional regulation, which included desensitization, internalization, down-regulation and recycling, of the wild type and mutated receptors. In order to do immunocytochemical studies, the epitope-tag receptors were also constructed. Signal transduction—Depending on the cell culture conditions, the Gq-protein coupled receptors can induce both growth stimulating and growth inhibitory effects. The recombinant adenovirus expressing dominant negative mutants were used to elucidate the pathway of signal transduction of the Gq-protein coupled receptors both in pancreatic cell lines and primary pancreatic acini cells. Bacterial and bacteriophage genetics—1) These studies utilized the approaches of molecular biology to study the expression and regulation of bacteriophage T4 ribonucleotide reductase, the T4-encoded nrdA and nrdB, genes. Both nrdA and nrdB genes were also cloned into a single expression vector to overproduce the functionally active ribonucleotide reductase and to investigate the role of this enzyme in the deoxyribonucleoside triphosphate synthetase multienzyme complex. 2) My laboratory also isolated and characterized xylanases from Bacillus firmus. We have cloned two alkaliphilic thermostable xylanase genes and characterized their chemo-physical properties and in the process of studying their regulation. Bioorganic chemistry--Chemical and enzymatic methods were used to synthesize oligopeptides and bioactive peptides. Several of biosynthetic enzymes were cloned by PCR from the genome of various bacteria and over-expressed them in E. coli or Bacillus sp. These enzymes were used to synthesize therapeutically agents.

調節幹原細胞及前驅細胞發育的因子:Notch傳導系統及細胞命運發育過程中,前驅細胞常接受環境的信息而演化成合宜的細胞系列。Notch是這些信息之一,Notch是穿過細胞膜的受體,在各式各樣的發育過程調控細胞的命運。Notch蛋白的功能既是細胞表面的受體也是基因轉錄的直接調控者。一般Notch訊息活化後,會抑制影響細胞系列的基因的轉錄,進而抑制細胞的分化,如此可調節細胞的命運。當Notch ligand與Notch受體蛋白的細胞外部分結合反應後,會促使全長的Notch受體蛋白被蛋白質分解脢在細胞內被切斷,而細胞內的部分,所謂活化型 Notch(NICD), 會轉移到細胞核內與DNA結合蛋白CBF1作用,來活化調控細胞分化的基因的轉錄。有許多調控蛋白可以和NICD-CBF1的複合體結合來促進或壓制這複合體的功能。另外Notch的訊息傳導也可經由不需CBF1調控蛋白的其他過程。Notch訊息傳導的分子機制是現在很熱門的研究話題。為了尋找影響Notch訊息傳導途徑之新調節因子並研究其作用機制,本實驗室已建立一個良好的實驗分析系統。我們正使用各樣的分子生物和蛋白體的技術來我們正使用各樣的分子生物和蛋白體的技術來尋找新調節因子。另外活化型Notch(NICD)對細胞的生理作用,尤其幹原細胞,也是本實驗室的重點。

專利 | Patent

U.S. patent 6,146,859; Patent entitled “Facile synthesis of L-homophenylanaline by equilibrium shift enzymatic reaction using tyrosine aminotransferase”, Co-inventor: 陳水田,蘇芳雅, 2000

論文與著作

  1. Sinchaikul, S., Pan, F.-M., Cheng, C.-W., Wang, C.-H., Fang, J.-M., Tseng, M.-J. & Chen, S.-T. (2005) Protein microarray using a-amino acids as metal tags on chips. Bioorg. Med. Chem. Lett. 15, 1413-1416.
  2. Yeh, T.-S., Hsieh, R.-H., Shen, S.-C., Wang, S.-H., Tseng, M.-J., Shih, C.-M. & Lin, J.-J. (2004) Nuclear bII- tubulin associates with the activated Notch receptor to modulate Notch signaling. Cancer Res. 64, 8334-8340.
  3. Chang, P., Tsai, W.-S., Tsai, C.-L. & Tseng, M.-J. (2004) Cloning and characterization of two thermostable xylanases from an alkaliphilic Bacillus firmus. Biochem. Biophys. Res. Commun. 319, 1017-1025.
  4. Yu, H.-M., Tseng, M.-J., Fang, J.-M., Phutrakul, S. & Chen, S. T. (2004) Capillary electrophoresis using immobilized whole cells with overexpressed endothelin receptor for specific ligand screening. Electrophoresis, 25, 1034-1041.
  5. Yeh, T.-S., Lin, Y.-M., Hsieh, R.-H. & Tseng, M.-J. (2003) Association of transcription factor YY1 with the high molecular weight notch complex suppresses the transactivation activity of Notch. J. Biol. Chem., 278, 41963-41969.
  6. Juan, H. F., Chang, W. H., Wu, C. Y., Pan, T. L., Tseng, M.-J., Khoo, K. H. & Chen, S. T. (2002) Biomic study of human myeloid leukemia cell line (HL-60) differentiated to macrophage using DNA array, proteomic, and bioinformatic analytical methods. Electrophoresis, 23, 2490-2504.
  7. Tseng, M.-J., Yap, M.-N., Ratanakhanokchai, K., Kyu, K. L. & Chen, S.-T. (2002) Purification and characterization of two cellulase free xylanases from an alkaliphilic Bacillus firmus. Enzyme Microbial Technology, 30,590-595.
  8. Chu, P.-W., Yap, M.-N., Wu, C.-Y., Huang, C.-M., Pan, F.-M., Tseng, M.-J. & Chen, S.-T. (2000) A proteomic analysis of secreted proteins from xylan-induced Bacillus sp. strain K-1. Electrophoresis, 21, 1740-1745.
  9. Tseng, M.-J. (2000) Phage-display libraries. Chemistry, 58, 191-200. (review, in Chinese)
  10. Yu, H.-M., Lin, H.-L., Wu, C.-Y., Tseng, M.-J., Chen, S.-T., Jeyashoke N. & Krisangkura, K. (1999) Enzymatic reaction in supercritical fluid carbon dioxide using dry-ice. J. Chin. Chem. Soc., 46, 647-650.
  11. Yu, H.-M., Chen, S.-T., Tseng, M.-J., Chen, S.-T. & Wang, K.-T. (1999) Microwave-assisted heterogeneous benzil-benzilic acid rearrangement. J. Chem. Res. (S) 62-63.
  12. Lin, I.-C., Sookkheo, B., Phutrakul, S., Chen, S.-T., Tseng, M.-J. & Wang, K.-T. (1999) Combinatorial peptide library for probing the selectivity of the s-1 subsite of proteases. J. Chin. Chem. Soc., 46, 147-152.
  13. Nicke, B., Tseng, M.-J., Fenrich, M. & Logsdon, C.D. (1999) Adenovirus-mediated gene transfer of RasN17 inhibits specific CCK actions on pancreatic acinar cells. Am. J. Phsiol., 276, (Gastrointest. Liver Physiol. 39) G499-506.

Presentations

  1. Li, C.J., Chung, S.C., Chen, H.–R., Tseng, M.-J. & Hwang, J. (2005) Generation of anti-cytokine antibodies for diagnosis of disease. Thirteenth Symposium on Recent Advances in Cellular and Molecular Biology (細胞及分子生物新知研討會), P. 71.
  2. Shih, P.-A., Tan, C.-Y., Tseng, M.-J., Chen, H.–R. & Hwang, J. (2005) Vaccination against severe respiratory syndrome (SARS) with the linear array epitopes (LAE) of different peptide regions on SARS-CoV spile protein. Thirteenth Symposium on Recent Advances in Cellular and Molecular Biology (細胞及分子生物新知研討會), P. 72.
  3. Lin, Y.-F., Tseng, M.-J., Hsu, H.-L., Wu, Y.-W., Lee, Y.-H. & Tsai, Y.-H. (2005) A novel FSH-activated signaling pathway: FSH-induced Gah/PLC-d1 activation mediating Ca2+-influx in rat Sertoli cells. Thirteenth Symposium on Recent Advances in Cellular and Molecular Biology (細胞及分子生物新知研討會), P. 291.
  4. Lee, Y.-C., Tseng, M.-J., Hu, C.-J., Leu, S.-J., Chiu, W.-T., Hsieh, M.-S. & Yang, Y.-Y. (2004) An immunodominant epitope is mapped to the N-terminus of nucleoprotein of severe acute respiratory syndrome coronavirus (SARS-CoV). 台北醫學大學九十二學年度師生聯合學術發表會, A-57.
  5. Lin, Y.-F., Tseng, M.-J. & Tsai Y.-H. (2003) Protein kinase C-mediated signaling pathway induce the maturation of human monocyte-derived dendritic cells. 第18th屆生物醫學聯合學術年會.
  6. Tseng, M.-J. (2002) Search for cellular proteins targeted by the intracellular Notch domain. 16th FAOBMB Symposium. Taipei, September 20-22.
  7. Tseng, M.-J. (2002) 生物晶片. 醫事檢驗公會繼續教育研習會-臨床檢驗診斷&醫學診斷新知. 苗栗為恭紀念醫院. July 27.
  8. Tseng, M.-J. (2002) Search interacting proteins by proteome. Rice Proteomics Workshop. Rice Research Center, Pathumthani, Thailand. July 9-12.
  9. Tseng, M.-J. (2002) Search interacting proteins by proteomic tools. Proteomics and Liver Disease Symposium. 中央研究院生物化學研究所. April 16-19.
  10. Tseng, M.-J. (2002) Study protein-proteininteraction by proteomic tools. 生物資訊研討會-蛋白體,臺灣生物資訊學會,臺灣健康暨管理學院。March 21.
  11. Lin, Y.-F., Tseng, M.-J. & Tsai Y.-H. (2002) The involvement of protein kinase C-mediated signaling pathway(s) in the differentiation processes of human monocyte-derived dendritic cells. 第17th屆生物醫學聯合學術年會, O38.
  12. Lin, Y.-F., Tseng, M.-J. & Tsai Y.-H. (2002) The pivotal role of protein kinase C-in human monocyte-derived dendritic cell differentation. 台北醫學大學九十一學年度師生聯合學術發表會.
  13. Tsai, M.-H., Yang. C.-C., Chen, P.-C., Yeh, T.-S. Tseng, M.-J. & Shih, D. T. (2001) Studies on the hematopoietic signal pathway in fetal liver across gestation. 台北醫學大學八十九學年度師生聯合學術發表會.
  14. Tsai, C.-L. & Tseng, M.-J. (2001) DNA shuffling of a xylanase gene from Bacillus subtilis. 台北醫學大學八十九學年度師生聯合學術發表會.
  15. 曾明中,謝銘隆,曾銘仁(Tseng, M.-J.),朱延和(2000)藉連續式紫外可見光分析法進行VanX酵素受質分子庫之篩選及生物活性測定. 中國化學會年會.
  16. Chang, W.-H. & Tseng, M.-J. (2000) The members of Notch signaling system in acute promyelocyte leukemia (APL) cell lines. 台北醫學大學八十八學年度師生聯合學術發表會.