國立中正大學 生命科學系 | 分子生物研究所 | 生物醫學研究所 專任師資

  • 張德卿
  • 蕭光明
  • 黃憲斌
  • 曾銘仁
  • 陳浩仁
  • 黃敏郎
  • 蕭淑惠
  • 江明格
  • 呂昱瑋
  • 李沁
  • 戴建國
  • 吳淑芬
  • 周正中
  • 陳永恩
  • 李政怡

  • 周正中 Cheng-Chung Chou

    168, University Road, Min-Hsiung Chia-Yi Taiwan, R. O. C.
    Tel: (05) 2720411 ext. 66506(Office) , 61517(Lab)
    Fax: (05) 2722871
    Email: bioccc@ccu.edu.tw

    • Lab

      奈米生醫分子診斷實驗室

    • Education

      Ph.D. Dept. of Chemical Engineering, Univ. of Maryland-College Park

    • Professional Experience

      95/08 ~ Present　助理教授　　　國立中正大學生命科學系暨分子生物研究所暨生物醫學研究所
      96/01 ~ Present　副研究員　 國家生技醫療產業策進會

    • Speciality

      (1)Nano-biotechnology
      (2)Molecular diagnostics
      (3) Biochips
      (4) Bioinformatics
      (5) Systems biology
      (6) Genomics

    • Research Project

      「奈米物質毒性評估平台的開發與建立」
      　　 ─　主持人　[奈米國家型科技計畫(98/08~101/07)]，計畫金額:27,500,000。

    • 「全新肺結核及其抗藥性檢驗試劑之開發(4)」
      　　 ─　主持人　[國家生技醫療產業策進會和衛生署疾病管制局(99/01~99/12)]，計畫金額:1,530,000。

      「全新禽流感及新型流感快速檢驗試劑之開發 (96-2321-B-194-001-MY2)」
      　　 ─　主持人　[國科會禽流感及新型流感計畫(96/08~98/07)]，計畫金額:5,600,000。
      「生技產業數位化資源共享平台建置開發III (96-3112-B-194-002-)」
      　　 ─　共同主持人　[基因體醫學國家型產學合作計畫 (96/05~97/04)]，計畫金額:7,000,000。
      「全新肺結合及其抗藥性檢驗試劑之開發(3/3)」
      　　 ─ 主持人　[國家生技醫療產業策進會和衛生署疾病管制局 (98/01~98/12)]，計畫金額:1,800,000。
      「全新肺結合及其抗藥性檢驗試劑之開發(2/3)」
      　　 ─ 主持人　[國家生技醫療產業策進會和衛生署疾病管制局 (97/01~97/12)]，計畫金額:2,200,000。
      「全新肺結合及其抗藥性檢驗試劑之開發(1/1)」
      　　 ─ 主持人　[國家生技醫療產業策進會和衛生署疾病管制局 (96/01~96/12)]，計畫金額:1,500,000。
      「探討巨噬細胞、支氣管表皮細胞和T細胞彼此間交互作用如何調控奈米碳管所引起的細胞毒性的分子機制 (96-2314-B-194-001-)」
      　　 ─　主持人　[國科會專題計畫(96/08~97/07)]，計畫金額:500,000。
      「發展基因網路建構方法以探討奈米碳管在人類肺臟形成肉芽腫的分子機制 (95-2312-B-194-003-)」
      　　 ─　主持人　[國科會專題計畫(95/12~96/07)]，計畫金額:440,000。
      「智慧型隨身氣喘偵測訊號研究」
      　　 ─　共同主持人　[台大與工研院合設奈米中心(95/01~95/12)]，計畫金額:1,000,000。

    • Research Description

      With interdisciplinary background on engineering, molecular biology, genomics/bioinformatics and mathematical/statistical modeling, three research fields are being pursued in this lab: nanobiotechnology, molecular biosensing, and systems biology.

      Nanobiotechnology. In this research area, several experimental skills such as virology, recombinant protein expression, nanotoxicity, nanoparticle labeling and nucleic acid technology will be integrated to tackle the following topics:

    • 1.Nanotoxicogenomics (奈米粒子毒性評估平台的開發與建立) To establish the macrophage/epithelial cell co-culture system for cytotoxicity assessment of nanomaterials (建立巨噬細胞/上皮細胞體外共同培養系統成為奈米材料毒性評估平台)

        To identify a set of key universal toxic genes induced in co-cultured macrophages exposed to widely-used nanomaterials and establish the gene expression profiling database (使用基因晶片找出巨噬細胞暴露在各種常見奈米材料所誘導產生具指紋辨識性的毒性基因羣並建立它們相對應的基因表達頻譜資料庫)

      To compare the toxic gene expression profiling of an unknown nanomaterial with the universal signature database for potential toxicity prediction of the nanomaterial (藉由比對新奈米材料所產生指紋毒性基因表達頻譜的變化來預測該奈米材料的可能毒性)

      2.Nanomedicine (奈米生醫應用)

      • To develop novel nanoparticle-virus complex delivery system for drug delivery and targeting in vitro and in vivo.
      • To further establish nanomotor-virus complex delivery system for accelerating molecular drug delivery

      Molecular biosensing. In this area, highly sensitive biosensors will be developed to detect two highly pandemic infectious pathogens, influenza virus (流感病毒) and M. tuberculosis (結核桿菌).

      1. Influenza virus detection

      • To develop quantum dot-induced fluorescence resonance energy transfer (FRET) reporter platform for real-time multiplex detection of different subtypes of influenza viruses (H1, H3, H5, and N1)
      • To develop nanogold-based oligonucleotide-aptamer test strip for visual identification of different influenza viral subtype (H1, H3, H5, and N1)

      2. Tuberculosis detection

      • To develop molecular beacon-based fluorescence in-situ hybridization assay for direct identification of M. tuberculosis.
      • To develop aptamer/protein/antibody array detection system for latent TB diagnosis from clinical blood samples.

      Systems biology. In this area, high-throughput measurement and analytical tools (e.g., DNA/protein/antibody/cell/siRNA microarrays and bioinformatics) will be used to obtain large-scale biological parameters for elucidating biological problems from a systems perspective. The emerging field of systems biology represents an integration of concepts and idea from life sciences, engineering disciplines and computer science.

      To develop biological pathway analysis framework for deciphering molecular toxic mechanism based on genome-wide microarray data (發展生物pathway分析平台從龐大基因晶片數據中解析出奈米材料暴露時可能造成細胞毒性的分子機制)

    To develop mathematical modeling to systematically discover novel functional modules and interactions in regulatory and metabolic networks

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    • Publications

      Article

      • Chou CC*, et al. Sandwich hybridization assay with a QD-induced FRET reporter system on an ITO glass slide: Toward H5N1 avian influenza virus detection, revised. (*Corresponding author)
      • Chen LC, Kao CF, Lao YH, Li YS, Liang JY, Chou CC*. Sandwich hybridization assay with a QD-induced FRET reporter system on an ITO glass slide: Toward H5N1 avian influenza virus detection. 2009; Biosensors & Bioelectronics (revised, SCI IF=5.143).
        • (*Corresponding author)
      • Chou CC, Hsiao HY, Hong QS, Chen CH, Peng YW, Chen HW and Yang PC. Single-walled carbon nanotubes can induce pulmonary injury in mouse model. Nano Letters, 2008;8:437-45. (SCI IF=10.371)
      • Chou CC and Peck K. Design and fabrication of spotted long oligonucleotide microarrays for gene expression analysis in Chapter 10 of Methods in Molecular Biology – Microarrays: Synthesis methods 2007; 381: 213-25 (book chapter)
      • Sher YP, Chou CC, Chou RH, Wu HM, Chang WSW, Chen CH, Yang PC, Wu CW, Yu CL, and Peck K. Human kallikrein 8 suppresses cancer cell invasiveness to yield favorable clinical outcome in non-small cell lung cancer. Cancer Research 2006; 66:11763-70. (SCI IF=7.656)
      • Chen HW, Su SF, Chien CT, Lin WH, Yu SL, Chou CC, Chen JW and Yang PC. Titanium dioxide nanoparticles induce emphysema-like lung injury in mice. FASEB Journal 2006; 20:E1732-412393-95. (SCI IF=6.721)
      • Chou CC, Lee TT, Chen CH, Hsiao HY, Lin YL, Ho MH, Yang PC and Peck K. Design of microarray probes for virus identification and detection of emerging viruses at the genus level. BMC Bioinformatics 2006; 7:232. (SCI IF=4.958)
      • Chen YA, Chou CC, Lu X, Slate EH, Peck K, Xu W, Voit EO and Almeida JS. A Multivariate prediction model for microarray cross-hybridization. BMC Bioinformatics 2006; 7: 101. (SCI IF=4.958)
      • Chou CC, Chen CH, Lee TT and Peck K. Optimization of probe length and the number of probes per gene for optimal microarray analysis of gene expression. Nucleic Acids Research 2004; 32:e99. (SCI IF=6.317)
      • Lo HR, Chou CC, Wu TY, Yuen JPY, and Chao YC. Novel baculovirus DNA elements strongly stimulate activities of exogenous and endogenous promoter. Journal of Biological Chemistry 2002; 277:5256-64. (SCI IF=5.808)

      Technique Report

      • 周正中。2005。Microarray data analysis。Formosan Journal of Medicine, Vol. 9, No.5, p622-627.
      • 周正中,白果能。2003。微陣列生物晶片。微機電系統技術與應用，第13章第2節，生物微機電系統：1117-1120頁。國科會精密儀器發展中心。
      • 周正中,白果能。2002。微陣列生物晶片簡介及其應用。科儀新知，第127期：5-13頁。
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    • Patent and Technology Transfer

      (I) Technology Transfer

      • Technology name: Database and probe sets for constructing transcript specific and tissue maker microarrays of sequenced organisms
        Authorization agent: Academia Sinica
        Inventors: 白果能(Konan Peck) and周正中(Cheng-Chung Chou)
        Acceptors: University of Connecticut, U.S.A. , 臺灣大學醫學院微陣列基因體醫學核心實驗室

      (II) Patent Filing

      • “Applications of molecular beacon imaging - pharmacogenomics”,
        PCT countries and USA provisional patent application 4907/16957-52501 (filed on 12/22/2006). Augustine Lin, PC Yang, CC Chou.
      • “Method of molecular beacon for detecting certain diseases”,
        PCT countries and USA provisional patent application 4907/16957-52500 (filed on 12/22/2006). Augustine Lin, PC Yang, CC Chou.
      • "Methods and applications of molecular beacon imaging for infectious disease and cancer detection",
        Taiwan patent application (filed on 12/25/2006). Augustine Lin, 楊泮池, 周正中.
      • "Merhods and applications of molecular beacon imaging for identifying and validating genomic targets and drug screening”
        Taiwan patent application (filed on 12/25/2006). Augustine Lin, 楊泮池, 周正中.
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    • 專利(含申請)

                • 1.周正中等,「可溫控之微型生物分子反應晶片」, Taiwan patent #: 98004 (2010)。
                • 2.羅仁權、周正中等,「自搜尋擴充與分類修正之智慧財產權媒合系統與方法」, Taiwan patent application (2009).
                • 3.Augustine Lin, PC Yang, CC Chou. Methods and applications of molecular beacon imaging for infectious disease and cancer detection. USPTO Application #: 20080032282, European Patent Application #: EP1979491, PCT Paten application #: PCT/US2006048853 (filing on 12/22/2006).
                • 4.Augustine Lin, 楊泮池, 周正中. Methods and applications of molecular beacon imaging for identifying and validating genomic targets and drug screening. Taiwan patent application (filinf on 12/25/2006).
 

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