YU-WEI, LEU

 Tel: (05)2720411 ext. Office 66507 Lab 61518

 Fax: (05)2722871

 Email: bioywl@ccu.edu.tw

• Ph.D. 1993-2001 Intercollege Genetic program, The Pennsylvania State University, University Park, PA, USA.
• B.S. 1984-1988 Department of Biology, TungHai University, Taichung, Taiwan.

• 2012- Associate Professor, Department of Life Science and Institute of Molecular Biology, National Chung-Cheng University, Chia-Yi, Taiwan.
• 2004-2012 Assistant Professor, Department of Life Science and Institute of Molecular Biology, National Chung-Cheng University, Chia-Yi, Taiwan.
• 2003-2004 Postdoctoral Research Associate, Human Cancer Genetics Program, The Ohio State University Comprehensive Cancer Center, Columbus, OH, USA (The whole lab moved from MO to OH).
• 2002-2003 Postdoctoral Research Associate, Department of Pathology and Anatomical Science, Ellis Fischel Cancer Center, Columbia, MO, USA.
• 2001-2002 Postdoctoral Research Associate, Ernest Gallo Clinic & Research Center, University of California, San Francisco, Emeryville, CA, USA.

Cancer & Functional Epigenomics

• 2008 6th National Innovation Award. 學術研究組 (2008「國家新創獎」) "量身定作的對位性基因體改造"
• 2009 7th National Innovation Award. 指導學生參加學生組(2009「國家新創獎」)"去氧核醣核酸甲基化活細胞觀測系統"

Genetics, Cancer, Epigenomics.

1. Yu-Wei Leu, Alfred S.-L and Tim Hui-Ming Huang. (2006) Use of CpG island microarray to interrogate the cancer epigenome. Promoter and CpG island Microarrays (Winegarden NA, Takashi M, eds). DNA Press (ISBN: 0-9748765-6-9).

1. TERMINATING THE EXPRESSION OF A TARGET GENE IN A CELL BY A METHYLATED POLYNUCLEOTIDE. Yu-Wei Leu, Shu-Huei Hsiao and Tim H.-M. Huang. (USA) SPE-16177(PE-34984-AM)
2. DETERMINING THE BIOLOGICAL FUNCTION OF A TARGET GENE IN A CELL. Yu-Wei Leu, Shu-Huei Hsiao and Tim H.-M. Huang. (USA) SPE-16648(PE-35149-AM)
3. TERMINATING THE EXPRESSION OF A TARGET GENE IN A CELL BY A METHYLATED POLYNUCLEOTIDE. Yu-Wei Leu, Shu-Huei Hsiao and Tim H.-M. Huang. (Taiwan) Pending 97121718(2008/6/11)
4. DETERMINING THE BIOLOGICAL FUNCTION OF A TARGET GENE IN A CELL. Yu-Wei Leu, Shu-Huei Hsiao and Tim H.-M. Huang.(Taiwan) Pending 97121717 (2008/6/11)
5. IN VIVO MONITORING SYSTEM FOR DNA METHYLATION. Yu-Wei Leu, Shu-Huei Hsiao, Chia-Chen Hsu and Tim H.-M. Huang.(USA) Pending 12/702797 (2010/2/9)
6. IN VIVO MONITORING SYSTEM FOR DNA METHYLATION.(用於哺乳動物細胞的三元件基因表現報導系統及其應用) Yu-Wei Leu, Shu-Huei Hsiao, Chia-Chen Hsu and Tim H.-M. Huang. Pending (2010/2/9)

1. Pei-Chi Peggy Hou, Ching-Chu Yen, Kuan-Der Lee, Hao-Chun Chang, Chao-Min Cheng, Yi-Shiuan Lin, H. Sunny Sun, Yu-Sun Chang, Tim H.-M. Huang, Shu-Huei Hsiao, Shaw-Jenq Tsai, and Yu-Wei Leu (2010) Global Knock-Down of the Cancer Epigenome. Amer. Assoc. Cancer Res. 101st Annual meeting, Washington DC. USA. (Abstract number: 183)
2. Yi-Shiuan Lin, Yu-Wei Leu, Pei-Chi Peggy Hou, I-Wen Teng, Shi-Gang Wang, Yeng-Jeng Shaw, Kuan-Der Lee, Chia-Chen Hsu, Joyce Yu-Hung Hung, Chao-Min Cheng, H. Sunny Sun, Yu-Sun Chang, Tim H.-M. Huang, Shaw-Jenq Tsai, and Shu-Huei Hsiao (2010) Global Epigenomic Modification Platform. Amer. Assoc. Cancer Res. 101st Annual meeting, Washington DC. USA. (Abstract number: 4915)
3. Yu-Wei Leu, Pei-Yi Chu, Chien-Min Chen, and Shu-Huei Hsiao (2009) Ethanol-induced epigenetic modifications in rat brain and mesenchymal stem cells. Neuroscience 2009, the 39th Annual Meeting of Society for Neuroscience, Oct. 17-21. Chicago, IL, USA.
4. Wan-Chen Wang, Ting-Hao Chung, Yu-Chieh Su, Kuan-Der Lee, Yu-Wei Leu and Wen-Chien Lee (2009) Characterization of neuronal differentiation of human bone marrow mesenchymal stem cells. Asia Pacific Biochemical Engineering Conference 2009 (APBioChEC'09), Kobe, Japan. (Abstract number: 407072-1)
5. Shu-Huei Hsiao, Kuan-Der Lee, Min-Jen Tseng, Wei-Sheng Sun, Chia-Chen Hsu, Hao-Chun Chang, Yi-Chen Hung, Ching-Chu Yen, Yu-Chen Chou, Yu-Chih Lin, I-Wen Teng, Yein-Ling Lai, Kun-Tu Yeh, Shaw-Jeng Tsai, Hsiao-Fang Sunny Sun, Yu-Sun Chang, Tim H.-M. Huang and Yu-Wei Leu (2009) Targeted DNA Methylation in Cell Fate Determination, The 12th SCBA International Symposium, Society of Chinese Bioscientists in America, Academia Sinica, Taipei, Taiwan.
6. Yu-Wei Leu, I-Wen Teng, Kuan-Der Lee, Kun-Tu Yeh, Hao-Chun Chang, Pei-Chi Peggy Hou, Yi-Shiuan Lin, Cheng-Han Lu, Chung-Hao Chien, Shaw-Jenq Tsai, H. Sunny Sun, Yu-Sun Chang, Tim H.-M. Huang and Shu-Huei Hsiao. (2009) Targeted HIC1 and RassF1A methylation is sufficient to cause tumorigenesis and mesenchymal to epithelial transformation. Amer. Assoc. Cancer Res. 100st Annual meeting, Denver, CO. USA. (Abstract number: 5150)
7. Chia-Chen Hsu, Shu-Huei Hsiao, Shaw-Jenq Tsai, Tim H.-M. Huang and Yu-Wei Leu (2009) Real-time monitoring of DNA methylation changes in live cells. Amer. Assoc. Cancer Res. 100st Annual meeting, Denver, CO. USA. (Abstract number: 1313)
8. I-Wen Teng, Yu-Wei Leu, Kuan-Der Lee, Kun-Tu Yeh, Hao-Chun Chang, Pei-Chi Peggy Hou, Yi-Shiuan Lin, Cheng-Han Lu, Chung-Hao Chien, Shaw-Jenq Tsai, H. Sunny Sun, Yu-Sun Chang, Tim H.-M. Huang , Shu-Huei Hsiao (2009) Targeted HIC1 and RASSF1A methylation is sufficient to cause tumorigenesis and mesenchymal to epithelial transformation. Seventeenth Symposium on Recent Advances in Cellular and Molecular Biology (第17屆細胞及分子生物新知研討會-墾丁冬令營), Heng Chun, Ping Tung, Taiwan. (SCMB_004 4847_8d54, 壁報論文獎)
9. Yu-Wei Leu, Shu-Huei Hsiao, Kuan-Der Lee, Min-Jen Tseng, Wei-Sheng Sun, Hao-Chun Chang, Chia-Chen Hsu, Yu-Chin Lin, Chou-Yu Chen, Yi-Chen Hung, Ching-Chu Yen, Kun-Tu Yeh, Shaw-Jeng Tsai, Hsiao-Fang Sunny Sun, Yu-Sun Chang, and Tim H.-M. Huang (2007) Elements in Functional Epigenomic Modeling for Somatic Stem Cell Differentiation and Tumorigenesis. 2007 Symposium of Bioinformatics and Systems Biology in Taiwan.
10. Yu-Wei Leu, Shu-Huei Hsiao, Kuan-Der Lee, Min-Jen Tseng, Wei-Sheng Sun, Chia-Chen Hsu, Hao-Chun Chang, Yi-Chen Hung, Kun-Tu Yeh, Shaw-Jeng Tsai, Hsiao-Fang Sunny Sun, Yu-Sun Chang, and Tim H.-M. Huang. (2007) Bivalent DNA methylation in cell fate determination. 第二十三屆生物夏令營, 溪頭.
11. Yu-Wei Leu, Shu-Huei Hsiao, Kuan-Der Lee, Min-Jen Tseng, Wei-Sheng Sun, Chia-Chen Hsu, Hao-Chun Chang, Yi-Chen Hung, Kun-Tu Yeh, Shaw-Jeng Tsai, Hsiao-Fang Sunny Sun, Yu-Sun Chang, and Tim H.-M. Huang. (2007) Bivalent DNA methylation in cell fate determination. 第二十三屆生物夏令營, 溪頭.
12. Yu-Wei Leu, Shu-Huei Hsiao, Kuan-Der Lee and Min-Jen Tseng (2006) Epigenomic Association between Somatic Stem Cell Differentiation and Tumerogenesis. National Research Program for Genomic Medicine (NPRGM) progress meeting, Taipei, Taiwan.
13. Yu-Wei Leu, Shu-Huei Hsiao, Kuan-Der Lee and Min-Jen Tseng (2006) Epigenomic Association between Somatic Stem Cell Differentiation and Tumerogenesis. National Research Program for Genomic Medicine (NPRGM) progress meeting, Taipei, Taiwan.
14. Pearlly S. Yan, Venkataramu Chinnambally, Ashraf Ibrahim, Joseph C. Liu, Nils Diaz, Barbara Centeno, Frank Weber, Yu-Wei Leu, Charis Eng, Timothy J. Yeatman, Tim H.-M. Huang. (2006) Mapping geographic zones of cancer risk with epigenetic biomarkers in normal breast tissue. Amer. Assoc. Cancer Res. 97th Annual meeting, Washington DC.
15. Alfred S Cheng, Yu-Wei Leu, Mei Y Fan, Pearlly S Yan, Kenneth P Nephew, Tim H Huang. (2004) Use of chromatin landscaping microarray to detect histone modifications in estrogen-responsive promoters. Chromatin, Chromosomes and Cancer Epigenetics, American Association for Cancer Research. Waikoloa, Hawaii.
16. Yu-Wei Leu, Pearlly S. Yan, Meiyun Fan, Victor X. Jin, Joseph C. Liu, Susan H. Wei, Ramana V. Davuluri, Christoph Plass, Kenneth P. Nephew, and Tim H.-M. Huang. (2004) Triggering of specific DNA methylation by disrupting the estrogen signaling pathway. Third Annual Post Graduate and Graduate Research Day, the Ohio State University. Columbus, OH.
17. Victor X Jin, Sandya Liyanarachchi, Hao Sun, Ramana V Davuluri , Yu-Wei Leu, Tim H.-M Huang. (2004) A computational Strategy for genome-wide ananlysis of the ER target genes. Third Annual Post Graduate and Graduate Research Day, the Ohio State University. Columbus, OH.
18. Yu-Wei Leu, Pearlly S. Yan, Meiyun Fan, Victor X. Jin, Joseph C. Liu, Susan H. Wei, Ramana V. Davuluri, Christoph Plass, Kenneth P. Nephew, and Tim H.-M. Huang. (2003) Triggering of specific DNA methylation by disrupting the estrogen signaling pathway. Jensen Symposium. Cincinnati, Ohio.
19. Victor X. Jin, Yu-Wei Leu, Meiyun Fan, Kenneth P. Nephew, Tim H.-M. Huang, and Ramana V. Davuluri. (2003) A computational Strategy for genome-wide ananlysis of the ER target genes. Jensen Symposium. Cincinnati, Ohio.
20. Yu-Wei Leu, Farahnaz Rahmatpanah, Huidong Shi, Joseph C. Liu, Susan H. Wei, Pearlly S. Yan, Tim Hui-Ming Huang. (2003) Effects of RNA interference on DNA methyltransferase gene expression and DNA methylation. Amer. Assoc. Cancer Res. 94th Annual meeting, Washington DC.
21. Pearlly S. Yan, Farahnaz Rahmatpanah, Joseph C. Liu, Tim Hsiao, Andrew Hsiao, Yu-Wei Leu, Huidong Shi, Tim H.-M. Huang. (2003) Disparity of methylation distribution within the RASSF1A CpG island in breast cancer. Amer. Assoc. Cancer Res. 94th Annual meeting, Washington DC.
22. Yu-Wei Leu and Tim Hui-Ming Huang. (2003) Diagnostic arrays for epigenetic markers. Aspen Cancer Conference, Aspen, Colorado.

1. Yu-Wei Leu,† Pei-Yi Chu,† Chien-Min Chen, Kun-Tu Yeh, Yu Ming Liu, Yen Hui Lee, Shan Tsu Kuo, Shaw-Jenq Tsai, Tim H.-M. Huang and Shu-Huei Hsiao (2011) Early Life Ethanol Exposure Causes Long-Lasting Disturbances on Rat Mesenchymal Stem Cells by Epigenetic Modifications. ( The Journal of Pathology,  10-614R, under revision) (†contributed equally to this paper)
2. I-Wen Teng*, Pei-Chi Hou*, Kuan-Der Lee, Pei-Yi Chu, Kun-Tu Yeh, Victor X Jin, Min-Jen Tseng, Chi-Sheng Wu, Yu-Sun Chang, H. Sunny Sun, Shaw-Jenq Tsai, Tim H.-M. Huang, Kuen-daw Tsai, Shu-Huei Hsiao+ and Yu-Wei Leu+ (2011) HIC1 and RassF1A methylation causes tumorigenesis. ( Cancer Research  CAN-10-3418R2, under revision) (*contributed equally to this paper; ＋ co-correspondence).
3. Jia–Shing Chen, Ih-Jen Su, Yu-Wei Leu, Kung-Chia Young and H. Sunny Sun. (2011) Expression of T-Cell Lymphoma Invasion and Metastasis 2 (TIAM2) Promotes Proliferation and Invasion of Liver Cancer.  Int J Cancer.  (accepted, 3/22/2011, IJC-10-2064.R3)
4. Chia-Chen Hsu*, Yu-Wei Leu*,  Min-Jen Tseng, Kuan-Der Lee, Tzen-Yu Kuo, Jia-Yi Yen, Yen-Ling Lai, Yi-Chen Hung, Wei-Sheng Sun, Pei-Yi Chu, Chien-Min Chen, Kun-Tu Yeh, Pearlly S. Yan, H. Sunny Sun, Yu-Sun Chang, Shaw-Jenq Tsai, Tim H.-M. Huang, and Shu-Huei Hsiao (2011) Functional characterization of Trip10 in cancer cell growth and survival.  Journal of Biomedical Science,  18:12. (*contributed equally to this paper).
5. Yi-Shiuan Lin, Arthur Y. Shaw, Shi-Gang Wang, Chia-Chen Hsu, I-Wen Teng, Min-Jen Tseng, Tim H.-M. Huang, Ching-Shih Chen, Yu-Wei Leu*  and Shu-Huei Hsiao* (2011) Identification of novel DNA methylation inhibitors via a two-Component reporter gene system.  Journal of Biomedical Science,  18:3. (*co-correspondence).
6. Chia-Chen Hsu, Hsin-Pai Li, Yu-Hung Hung, Yu-Wei Leu,  Wu-Hsiung Wu, Feng-Sheng Wang, Kuan-Der Lee, Pey-Jium Chang, Chi-Sheng Wu, Yen-Jung Lu, Tim H.-M. Huang, Yu-Sun Chang and Shu-Huei Hsiao (2010) Targeted methylation of CMV and E1A viral promoters. Biochem. Biophys.  Res. Commun.,  402:228-234.
7. Shu-Huei Hsiao*, Kuan-Der Lee*, Chia-Chen Hsu, Min-Jen Tseng, Victor X. Jin, Wei-Sheng Sun, Yi-Chen Hung, Kun-Tu Yeh, Pearlly S. Yan, Yen-Yu Lai, H. Sunny Sun, Yen-Jung Lu, Yu-Sun Chang, Shaw-Jenq Tsai, Tim H.-M. Huang and Yu-Wei Leu (2010) DNA methylation of the Trip10 promoter accelerates mesenchymal stem cell lineage determination. Biochem.  Biophys. Res. Commun.,  400:305-312.
8. Shu-Huei Hsiao, Tim H.-M. Huang and Yu-Wei Leu (2009) Excavating relics of DNA methylation changes during the development of neoplasia.  Seminar in Cancer Biology,  19:198-208.
9. Yu-Wei Leu and Tim H.-M. Huang. Global DNA Methylation in the Modeling of Tumoral Progression.  Cell Science Reviews,  4, No. 2: 2007(ISSN1742-8130).
10. Pearlly S. Yan, Venkataramu Chinnambally, Ashraf Ibrahim, Joseph C. Liu, Nils Diaz, Barbar Centeno, Frank Weber, Yu-Wei Leu,  Charis Eng, Timothy J. Yeatman, Tim H.-M. Huang. Mapping Geographic Zones of Cancer Risk with Epigenetic Biomarkers in Normal Breast Tissue.  Clin Cancer Research,  63: 6626-6636, 2006.
11. Alfred S. Cheng, Victor X. Jin, Meiyun Fan, Laura T. Smith, Sandya Liyanarachchi, Pearlly S. Yan, Yu-Wei Leu,  Michael W. Chan, Christoph Plass, Kenneth P. Nephew, Ramana V. Davuluri, Tim H.-M. Huang. Combinatorial analysis of transcription factor partners reveals recruitment of c-MYC to ER-responsive promoter.  Mol. Cell,  21: 393-404, 2006
12. Hsin-Pai Li, Yu-Wei Leu and Yu-Sun Chang. Epigenetic changes in virus-associated human cancers.  Cell Research,  15: 262-271, 2005.
13. Yu-Wei Leu,  Pearlly S. Yan, Meiyun Fan, Victor X. Jin, Joseph C. Liu, Susan H. Wei, Ramana V. Davuluri, Christoph Plass, Kenneth P. Nephew, and Tim H.-M. Huang. Loss of estrogen signaling leads to epigenetic silencing of its target genes in breast cancer.  Cancer Research,  64:8184-8192, 2004.
14. Victor X Jin*, Yu-Wei Leu*,  Sandya Liyanarachchi, Hao Sun, Kenneth P. Nephew, Tim H.-M Huang, Ramana V Davuluri. Identifying estrogen receptor a target genes using integrated computational genomics and chromatin immunoprecipitation microarray.  Nucleic Acids Res,   32: 6627-6635, 2004. (*contributed equally to this paper)
15. Yu-Wei Leu,  Farahnaz Rahmatpanah, Huidong Shi, Joseph C. Liu, Susan H. Wei, Pearlly S. Yan, Tim Hui-Ming Huang. Double RNA interference of DNMT3b and DNMT1 enhances DNA demethylation and gene reactivation.  Cancer Research,  63: 6110-6115, 2003.
16. Huidong Shi, Susan Wei, Yu-Wei Leu,  Farahnaz Rahmatpanah, Joseph Liu, Pearlly Yan, Kenneth P. Nephew, Tim H.-M. Huang. Triple analysis of the cancer epigenome: an integrated microarray system for assessing gene expression, DNA methylation, and histon acetylation.  Cancer Research,  63: 2164-2171, 2003.
17. Pearlly S. Yan, Huidong Shi, Farahnaz Rahmatpanah, Tim Hsiao, Andrew Hsiao, Yu-Wei Leu,  Joseph C. Liu, Tim H.-M. Huang. Differential distribution of DNA methylation within the RASSF1A CpG island in breast cancer.  Cancer Research,  63: 6178-6186, 2003.
18. Yu-Wei Leu and Tim Hui-Ming Huang. Diagnostic arrays for epigenetic markers.  Toxicologic Pathology,  31 (1) 140-170, 2003.
19. Lu YJ, Wu CS, Li HP, Liu HP, Lu CY, Leu YW, Wang CS, Chen LC, Lin KH, Chang YS. (2010) Aberrant methylation impairs low density lipoprotein receptor-related protein 1B tumor suppressor function in gastric cancer.  Genes Chromosomes Cancer,  49(5): 412-24.
20. Wu CS, Lu YJ, Li HP, Hsueh C, Lu CY, Leu YW,  Liu HP, Lin KH, Huang TH, Chang YS (2010) Glutamate receptor, ionotropic, kainate 2 silencing by DNA hypermethylation possesses tumor suppressor function in gastric cancer.  Int J Cancer. 126: 2542-52.