CHENG-I, LEE 

 Tel: (05)2720411 ext. Office 66511 Lab 61523

 Fax: (05)2722871

 Email: biocil@ccu.edu.tw

B. S., Chemistry, National Tsing Hua University, Taiwan          1992 - 1996

M. S., Chemistry, National Tsing Hua University, Taiwan         1996 - 1998

Ph. D., Biophysical Chemistry, Yale University, USA               1999 - 2005

Post-Doctoral Associate, Medical Biotechnology Center, University of Maryland Biotechnology Institute, USA  2005 – 2007

Assistant Professor, Life Science, National Chung Cheng University, Taiwan  2007 – 2013

Associate Professor, Life Science, National Chung Cheng University, Taiwan       2013- 2017

Associate Professor, Biomedical Sciences, National Chung Cheng University  2017- 2019

Professor, Biomedical Sciences, National Chung Cheng University, Taiwan   2019- current

Chair Professor, Biomedical Sciences, National Chung Cheng University, Taiwan 2021/8-2024/7

• Biochemistry 生物化學
• Biophysics 生物物理
• Bioinorganic Chemistry 生物無機化學
• Bio-Nano Imaging 生物奈米影像  

Biochemical and biophysical studies on misfolding of prion proteins.
Nanoimaging of prion fibrils.

Prion diseases are progressive neurodegenerative disorders that affect both humans and animals, such as Creutzfeldt-Jakob disease (CJD) in humans, bovine spongiform encephalopathy (mad cow disease) in cows and Scrapie in sheep. Up to date, there is no therapeutic treatment to control or to cure prion diseases. Prion diseases are caused by accumulation of prion amyloid fibrils resulted from misfolding of normal α-helix rich prion proteins into β-sheet rich prion proteins. How prion monomers misfold and convert to fibrils is not know yet. We are interested in studying the mechanism of the prion fibril formation by molecular spectroscopy and nanoimaging.

Teaching award at National Chung Cheng University  國立中正大學教學優良獎

Young scholar award at National Chung Cheng University  國立中正大學青年學者獎

1. Cheng-Ping Jheng and Cheng-I Lee*, “Combination of structure-based virtual screening, molecular docking and molecular dynamics approaches for the discovery of anti-prion fibril flavonoids” Frontiers in Molecular Bioscience (2023) 9, 1088733 [SCI]. (doi: 10.3389/fmolb.2022.1088733).
2. Li-Shiun Chen, Cheng-I Lee, Yuan-Pern Lee, “Isomers of SO₂: Infrared absorption of SOO in solid argon”, Journal of Chemical Physics, (1996) 105, 9454-9460 [SCI].
3. Cheng-I Lee, Yuan-Pern Lee, Xuefeng Wang, Qi-Zong Qin, “Isomers of N₂O₃: Observation of trans-cis N₂O₃ in solid Ar”, Journal of Chemical Physics (1998) 109, 10446-10455 [SCI].
4. Sergei Vassiliev, Cheng-I Lee, Gary W. Brudvig, Doug Bruce, “Structure-based kinetic modeling of excited-state transfer and trapping in histidine-tagged Photosystem II core complexes from Synechocystis”, Biochemistry (2002) 41, 12236-12243 [SCI].
5. Cheng-I Lee, Gary W. Brudvig, “Investigation of the functional role of Ca²⁺ in the oxygen-evolving complex of Photosystem II: a pH-dependence study of the substitution of Ca²⁺ by Sr²⁺”, Journal of the Chinese Chemical Society (2004) 51, 1221-1228 [SCI].
6. Leonid Breydo, Ying Sun, Natallia Makarava, Cheng-I Lee, Vera Novitskaia, Olga Bocharova, Joseph P. Y. Kao, Ilia, V. Baskakov, “Nonpolar substitution at the C-terminus of the prion protein, a mimic of the glycosylphosphatidulinositol anchor, partially impairs amyloid fibril formation”, Biochemistry (2007) 46, 852-61 [SCI].
7. Cheng-I Lee, K. V. Lakshmi, Gary W. Brudvig, “Probing the functional role of Ca²⁺ in the oxygen-evolving complex of Photosystem II by metal ion inhibition”, Biochemistry (2007) 46, 3211-3223 [SCI].
8. Cheng-I Lee, Qingyuan Yang, Veronique Perrier, Ilia V. Baskakov, “The dominant negative effect of the Q218K variant of the prion protein does not require protein X”, Protein Science (2007) 16, 2166-2173 [SCI].
9. Natallia Makarava, Cheng-I Lee, Valeriy G. Ostapchenko, Ilia V. Baskakov, “Highly promiscuous nature of prion polymerization”, Journal of Biological Chemistry (2007) 282, 36704-36713 [SCI].
10. Ying Sun, Natallia Makarava, Cheng-I Lee, Pongpan Laksanalamai, Frank T. Robb, Ilia V. Baskakov, “Conformational stability of PrP amyloid fibrils controls their smallest possible fragment size.” Journal of Molecular Biology. (2008) 376, 1155-1167 [SCI].
11. 江素玉、李政怡、馮學深、蔡宛霖、羅祥文, “同步輻射圓二色光譜實驗站與應用”科儀新知 (2009), 30(5), 9-17.
12. Cheng-I Lee*, Nai-yuan Chang, “Characterizing the denatured state of human prion 121–230.” Biophysical Chemistry (2010) 151, 86-90 [SCI].
13. Barbara Yang, Kuen-Hua You, Shing-Chuen Wang, Hau-Ren Chen and Cheng-I Lee, “The Effects of Trimethylamine N-Oxide on the Structural Stability of Prion Protein.” Neurodegenerative Diseases, (2011) p311-326.
14. Jian-Chau Luo, Shing-Chuen Wang, Wei-Bang Jian, Chien-Hsing Chen, Jaw-Luen Tang and Cheng-I Lee*, “Formation of amyloid fibrils from b-amylase.” FEBS Letters, (2012) 586, 680-685 [SCI].
15. Jaw-Luen Tang, Po-Jen Wu, Shing-Chuen Wang and Cheng-I Lee*, “Insights into structural properties of denatured human prion 121-230 at melting temperature studied by replica exchange molecular dynamics.” Journal of Physical Chemistry-B, (2012) 116, 3305–3312 [SCI].
16. Li-Chen Yang, Yen-Shang Lai, Chin-Ming Tsai, Yi-Ting Kong, Cheng-I Lee,^* and Cheng-Liang Huang^*, “One-pot synthesis of monodispersed silver nanodecahedra with optimal SERS activities using seedless photo-assisted citrate reduction method.” Journal of Physical Chemistry-C, (2012) 116, 24292-24300 [SCI].
17. Wen-Tsai Ji, Cheng-I Lee, Jeff Yi-Fu Chen, Ya-Ping Cheng, Sheng-Ru Yang, Jung-Hua Chen1, Hau-Ren Chen*, “Areca Nut Extract Induces Pyknotic Necrosis in Serum-Starved Oral Cells via Increasing Reactive Oxygen Species and Inhibiting GSK3b: An Implication for Cytopathic Effects in Betel Quid Chewers.” PLOS One, (2013) 8, e63295 [SCI].
18. Ruchira Chatterjee, Christopher S. Coates, Sergey Milikisiyants, Cheng-I Lee, Arlene Wagner, Oleg G. Poluektov and K. V. Lakshmi*, “High-Frequency Electron Nuclear Double-Resonance Spectroscopy Studies of the Mechanism of Proton-Coupled Electron Transfer at the Tyrosine-D Residue of Photosystem II.” Biochemistry, (2013) 52, 4781-4790 [SCI].
19. Chi-Fen Lin, Kun-Hua Yu, Cheng-Ping Jheng, Raymond Chung* and Cheng-I Lee*, “Curcumin Reduces Amyloid Fibrillation of Prion Protein and Decreases Reactive Oxidative Stress.” Pathogens, (2013) 2, 506-519 [SCI].
20. Shen-Jie Lin, Kun-Hua Yu, Jhih-Ru Wu, Chin-Fa Lee, Cheng-Ping Jheng, Hau-Ren Chen and Cheng-I Lee*, “Liberation of GPI-Anchored Prion from Phospholipids Accelerates Amyloidogenic Conversion” International Journal of Molecular Science, (2013) 14, 17943-17957 [SCI].
21. Morris M. H. Hsu, Pei-Yu Huang, Yao-Chang Lee, Yuang-Chuen Fang, Michael W.Y. Chan, and Cheng-I Lee*, “FT-IR microspectrometry reveals the variation of membrane polarizability due to epigenomic effect on epithelial ovarian cancer” International Journal of Molecular Science, (2014) 15, 17963-17973 [SCI].
22. Jian-Liang Chou, Lin-Yu Chen, Sheng-Jie Lin, Pearlly S. Yan, Wei-Ting Chao, Cheng-I Lee, Kenneth P. Nephew, Tim H.-M. Huang, Hung-Cheng Lai* and Michael W.Y. Chan*, “Hypermethylation of a TGF-b target, ABCA1 is associated with poor prognosis in ovarian cancer patients” Clinical Epigenetics, (2015) 7, 1-11 [SCI].
23. Yi-Ci Li, Yu-Ting Kuo, Po-Yuan Huang, Sidney S. Yang, Cheng-I Lee*, and Tai-Huei Wei*, “Thermal Lensing Effect of CS2 Studied with Femtosecond Laser Pulses” submitted to Physical Chemistry Chemical Physics (2015) 17, 24738-24747 [SCI].
24. Li-Shu Lee, Yu-Ting Kuo, Chi-Chen Wang, Yi-Ci Li, Po-Yuan Huang, Cheng-I. Lee* and Tai-Huei Wei*, “Mechanism of short-pulse-induced solute migration in comparison to continuous-light-driven thermal diffusion” RSC Advances (2016) 6, 20671-20680 [SCI].
25. Nai-yuan Chang, Yi-Ci Li, Cheng-Ping Jheng, Yu-Ting Kuo and Cheng-I Lee*, “Characterizing the denatured state ensemble of ubiquitin under native conditions using replica exchange molecular dynamics” RSC Advances (2016) 6, 95584-95589 [SCI].
26. Yi-Ci Li, Yu-Ting Kuo, Po-Yuan Huang, Cheng-I Lee and Tai-Huei Wei*, “Ultrashort-laser-pulse-induced thermal lensing in pure H₂O and a NaCl-H₂O solution” RSC Advances (2016) 6, 112727-112737 [SCI].
27. Yi-Hsuan Hsieh, Jun-Hui Zhang, Wen-Ching Chuang, Kun-Hua Yu, Xian-Bin Huang, Yao-Chang Lee and Cheng-I Lee*, “Effect of Combined Treatment with Photodynamic and Chemical Therapies on Candida albicans” International Journal of Molecular Science (2018) 19, 337 [SCI].
28. Wen-Hsuan Tsai, Kun-Hua Yu, Yi-Cheng Huang, and Cheng-I Lee*, "EGFR-targeted photodynamic therapy by curcumin-encapsulated chitosan/TPP nanoparticles" International Journal of Nanomedicine, (2018) 13, 903-916 [SCI].
29. Yi-Hsuan Hsieh, Wen-Ching Chuang, Kun-Hua Yu, Cheng-Ping Jheng and Cheng-I Lee*, “Sequential photodynamic therapy with phthalocyanine encapsulated chitosan-tripolyphosphate nanoparticles and flucytosine treatment against Candida tropicalis” Pharmaceutics (2019) 11, 16 [SCI].
30. Huan-I Jen, Zih-You Lin, Jin-Xun Guo and Cheng-I Lee*, “The effects of divalent cation-chelated prion fibrils on the immune response of EOC 13.31 microglia cells” Cells (2020) 9, 2285 [SCI].
31. Kun-Hua Yu and Cheng-I Lee*, “Quercetin disaggregates prion fibrils and decreases fibril-induced cytotoxicity and oxidative stress” Pharmaceutics (2020) 12, 1081 [SCI].
32. Kun-Hua Yu, Mei-Yu Huang, Yi-Ru Lee, Yu-Kie Lin, Hau-Ren Chen and Cheng-I Lee*, “The effect of octapeptide repeats on prion folding and misfolding” International Journal of Molecular Science (2021) 22, 1800 [SCI].
33. Cheng-I Lee, Chu-Bin Liao, Chih-Shang Chen, Fen-Ying Cheng, Yu-Hsuan Chung, Yu-Chuan Wang, Sian-Yi Ciou, Wen-Yun Hsueh, Tzu-Hao Lo, Guan-Ru Huan, Hsin-Yi Huang, Chia-Shen Tsai, Yu-Jung Lu, Shih-Hsien Chuang, Jiann-Jyh Huang*, “Design and synthesis of 4-anilinoquinazolines as Raf kinase inhibitors. Part 1. Selective B-Raf/B-RafV600E and potent EGFR/VEGFR2 inhibitory 4-(3-hydroxyanilino)-6-(1H-1,2,3-triazol-4-yl)quinazolines” Bioorganic Chemistry (2021) 109, 104715 [SCI].
34. Kun-Hua Yu, Cheng-Ping Jheng and Cheng-I Lee*, “Quercetin binding accelerates prion fibrillation into proteinase sensitive and loosely structured amyloids” Biomedicine & Pharmacotherapy (2022) 151, 113177 [SCI].
35. Kun-Hua Yu, Cheng-Ping Jheng and Cheng-I Lee*, “Quercetin binding accelerates prion fibrillation into proteinase sensitive and loosely structured amyloids” Biomedicine & Pharmacotherapy (2022) 151, 113177 [SCI]. (doi: 10.1016/j.biopha.2022.113177)
36. Dhivyabharathi Balakrishnan and Cheng-I Lee*, “Surface functionalization of bamboo with silver-reduced graphene oxide nanosheets to improve hydrophobicity and mold resistance” Coatings (2022) 12, 980 [SCI].