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Hsien-Bin Huang

Hsien-Bin Huang

Contact  information

 Tel: (05)2720411 ext. Office 53200 Lab 53201

 Fax: (05)2722871

 Email: biohbh@ccu.edu.tw

Academic qualication
  • 1979-1984 B.S. Chemistry National Taiwan Normal University
  • 1984-1986 M.S. Biochemistry National Taiwan University
  • 1987-1992 phD. Biochemistry Cornell University Ph.D.
Specialties
  • Protein Neuroscience
  • Enzymology
  • Protein Chemistry
Current position

Professor, Department of Life Science/Institute of Molecular Biology National Chung Cheng University

Working Experience
  • Aug. 2003-2005 Chairman, Department of Life Science, National Chung Cheng University.
  • Aug. 2008-present Professor, Department of Life Science, National Chung Cheng University.
  • Aug. 2000-2008 Associate Professor, Institute of Molecular Biology, National Chung Cheng University.
  • Aug. 1995-July 2000 Associate Professor, Division of Biochemistry, Tzu Chi University.
  • Jan. 1992-July 1995 Postdoctor, Lab. of Molecular and Cellular Neuroscience, The Rockefeller University
  • (Advisor: Paul Greengard, The Noble Prize Laureate in 2000 for medicine and physiology)
Research area 

Protein Neuroscience, Enzymology, Protein Chemistry

My research is concerned with the study of proteins, especially those involved in neuronal functions. The research involved isolation of proteins, determination of their structures and properties, cloning, and expression. The research is basic in nature, but significant relationships between this research and human disease. The research projects include two parts. First is focused on the interactions between the protein phosphatase-1 (PP1) and its regulatory proteins, which play a key role in dopaminergic signal transduction of the brain. My laboratory has identified the mechanism of PP1 regulated by one of its inhibitors, inhibitor-2. Recent studies have led to the elucidation of the first secondary structure of inhibitor-2 by NMR. Our final goals are to better understand the molecular mechanism in regulation of PP1, which may result in the design of drugs capable of mimicking or antagonizing the action of PP1, and the role of PP1 in regulation of neuronal functions. Purification and characterization of the novel PP1-binding proteins will be also included. A second area of research created recently concerns the mechanism in prevention of Ab polymerization and the structural differences of apoE isoforms, both of which are highly associated with the pathogenesis of the Alzheimer's disease (AD). Recently, we have planed to design the photo-sensitive peptides that can block the Ab polymerization. Our aim is to use NMR to elucidate 3-dimensional structure of Ab:peptide complex, providing the targets for design of therapeutic drugs in preventing the development of AD. Three common alleles of human apoE, e2, e3 and e4, are found, differing by a single amino acid substitution in residue-112 and –158 and showing the remarkable differences in their biochemistry, and cellular metabolism and biological functions. In this project, we focus on the structural studies of apoE isoforms by using NMR (collaborated with Dr. Ta-Hsien Lin, at VGH, Taipei). Our goals are to understand the relationship between protein structure and function.

Archievement
  • Research Award (甲等獎) (1997, 1999, National Science Council)
  • Outstanding Research Award (2005, National Chung Cheng University)
  • 中正大學教師優良教學獎 (2007)
  • 慈濟佛教大林分院顧問 (2007-present)
Publications
  1. Lai NS, Yu HC, Huang Tseng HY, Hsu CW,  Huang HB, Lu MC (2021) Increased serum levels of brain-derived neurotrophic factor contribute to inflammatory responses in patients with rheumatoid arthritis.  Int. J. Mol. Sci.   22, 1841; doi.org/10.3390/ijms22041841.
  2. Yu HC*, Huang KY, Lu MC, Huang Tseng HY, Liu SQ, Lai NS*,  Huang HB* (2021) Down-regulation of LOC645166 in T cells of ankylosing spondylitis patients promotes the NF-κB signaling via decreasingly blocking recruitment of the IKK complex to K63-linked polyubiquitin chains.  Front. Immunol.   12, 5917056.
  3. Yu HC, Tung CH, Huang KY,  Huang HB, Lu MC (2020) The essential role of peptidylarginine deiminases 2 for cytokines secretion, apoptosis, and cell Adhesion in macrophage.  Int. J. Mol. Sci.   21, 5720; doi:10.3390/ijms21165720.
  4. Lin YS1, Huang KY1, Yu HC1, Lu MC, Fan CJ, Huang Tseng HY, Jhuang BY,Liu SQ, Lai NS*, Lin TH*,  Huang HB* (2020) Identification of phostensin in association with Eps 15 homology domain-containing protein 1 and 4.  Biochem.Biophys. Res. Commum.   531, 236-241.
  5. He KC, Chen YR, Liang CT, Huang SJ, Tzeng CY, Chang CF, Huang SJ,  Huang HB*, Lin TH* (2020) Conformational characterization of native and L17A/F19A- substituted Dutch-type β-amyloid peptides.   Int. J. Mol. Sci.   21, 2571.
  6. Li SC, Lee CC, Hsu CM,  Huang HB,Su YC (2019) IL-6 induces haptoglobin expression through activating STAT3 in human head and neck cancer.  J. Oral Pathol. Med.   doi: 10.1111/jop.12958.
  7. Lai NS, Yu HC, Tung CH, Huang KY,  Huang HB, Lu MC (2019) Increased peptidylarginine deiminases expression during the macrophage differentiation and participated inflammatory responses.  Arthritis Res.Ther.   21:108.
  8. Lai NS, Yu HC, Huang KY, Tung CH,  Huang HB, Lu MC (2018) Decreased T cell expression of H/ACA box small nucleolar RNA 12 promotes lupus pathogenesis in patients with systemic lupus erythematosus.  Lupus   7: 1499-2508.
  9. Lai NS, Yu HC, Tung CH, Huang KY,  Huang HB, Lu MC (2018) Aberrant expression of interleukin-23-regulated miRNAs in T cells from patientswith ankylosing spondylitis.  Arthritis Res. Ther.   20:259.
  10. Liang CT, Lin YS, Huang YC, Huang HL, Yang JQ, Wu TH, Chang CF, Huang SJ,  Huang HB,* Lin TH* (2018) Characterization of the interactions between inhibitor-1 and recombinant PP1 by NMR spectroscopy.  Sci. Rep.   8: 50.
  11. Yu HC, Huang KY, Liu MC,Huang HL, Liu SQ, Lai NS,*,  Huang HB* (2017) Targeted delivery of the HLA-B*27-binding peptide into the endoplasmic reticulum suppresses the IL-23/IL-17 axis of immune cells in spondylarthritis.  Mediat. Inflamm.   207: 4016802
  12. Liu WT, Huang KY, Lu MC, >Huang HL, Chen CY, Cheng YL, Yu HC, Liu SQ, Lai NS*,  Huang HB* (2017) TGF-β up-regulates the translation of USP15 via the PI3K/AKT pathway to promote p53 stability.  Oncogene   36: 2715-2723.
  13. Yu HC, Lai PH, Lai NS,  Huang HB, Koo M, Lu MC (2016) Increased Serum Levels of Anti-Carbamylated 78-kDa Glucose-Regulated Protein Antibody in Patients with Rheumatoid Arthritis.  Int. J. Mol. Sci.  17: 1510.
  14. Yin, WY, Lee MC, Huang HB, Lu MC (2016) Increased gene expression of TGF-β in peripheral blood mononuclear cells from renal transplant patients with polyomavirus BK viremia.  Clin. Transplant.  30: 393-398.
  15. Lu MC, Yu HC, Yu CL,  Huang, HB, Koo M, Tung CH, Lai NS (2016) Increased expression of long noncoding RNAs LOC100652951 and LOC100506036 in T cells from patients with rheumatoid arthritis facilitates the inflammatory responses.   Immunol. Res.   64: 576-583.
  16. Liang CT1,  Huang HB1, Wang CC, Chen YR, Chang CF, Shiao MS, Chen YC, Lin TH (2016) L17A/F19A substitutions augment the α-helicity of β-amyloid peptide discordant segment.  PLoS ONE   11: e0154327.
  17. Yu CC,  Huang HB, Hung SK, Liao HF, Lee CC, Lin HY, Li SC, Ho HC, Hung CL, Su YC (2016) AZD2014 radiosensitizes oral squamous cell carcinoma by inhibiting AKT/mTOR axis and inducing G1/G2/M cell cycle arrest.  PLoS ONE   11(3): e0151942.
  18. Lu MC, Yu CL, Yu HC,  Huang HB, Koo M, Lai NS* (2016) Anti-citrullinated protein antibodies promote apoptosis of mature human Saos-2 osteoblasts via cell-surface binding to citrullinated heat shock protein 60.  Immunobiology   221, 76–83.
  19. Yu HC, Lu MC, Huang KY, Huang HL, Liu SQ,  Huang HB*, Lai NS* (2016) Sulfasalazine treatment suppresses the formation of HLA-B27 heavy chain homodimer in patients with ankylosing spondylitis.  Int. J. Mol. Sci.   17, 46.
  20. Lai NS, Yu HC, Yu CL, Koo M,  Huang HB, Lu MC* (2015) Anti-citrullinated protein antibodies suppress let-7a expression in monocytes from patients with rheumatoid arthritis and facilitate the inflammatory responses in rheumatoid arthritis.  Immunobiology  220, 1351–1358.
  21. Yu HC, Huang KY, Lu MC, Huang HL, Liu WT, Lee WC, Liu SQ,  Huang HB* , Lai NS* (2015) Characterization of the Recognition Specificity of BH2, a Monoclonal Antibody Prepared against the HLA-B27 Heavy Chain.  Int. J. Mol. Sci.   16, 8142-8150.
  22. Lo CJ, Wang CC,  Huang HB, Chang CF, Shiao MS, Chen YC, Lin TH (2015) The Arctic mutation accelerates Aβ aggregation in SDS through reducing the helical propensity of residues 15–25.  Amyloid   22, 8-18.
  23. Lo CJ, Chyan CL, Chen YC, Chang CF,  Huang HB*, Lin TH* (2015) Resonance assignments and secondary structure of apolipoprotein E C-terminal domain in DHPC micelles.  Biomol. NMR Assign.   9, 187-190.
  24. Lu MC, Yu CL, Chen HC, Yu HC,  Huang HB, Lai NS (2015) Aberrant T cell expression of Ca2+ influx-regulated miRNAs in patients with systemic lupus erythematosus promotes lupus pathogenesis.   Rheumatol.   54, 343-348.
  25. Huang SH, Ke SC, Lin TH,  Huang HB, Chen YC (2014) Effect of C-terminal residues of Aβ on copper binding affinity, structural conversion and aggregation.  PLoS One  9(3), e90385.
  26. Yu CC, Hung SK, Liao HF, Lee CC, Lin HY, Lai HC, Li SC, Ho HC,  Huang HB*, Su YC* (2014) RAD001 Enhances the radiosensitivity of SCC4 oral cancer cells by inducing cell cycle arrest at the G2/M checkpoint.   Anticancer Res.   34, 2927-2935.
  27. Lin YS, Huang HL, Liu WT, Lin TH*,  Huang HB* (2014) Identification of the high molecular weight isoform of phostensin.   Int. J. Mol. Sci.  15, 1068-1079.
  28. Lai NS, Yu HC, Chen HC, Yu CL,  Huang HB , Lu MC (2013) Aberrant expression of microRNAs in T cells from patients with ankylosing spondylitis contributed to the immunopathogenesis.  Clin. Exp. Immunol.  173, 47-57.
  29. Tseng KC, Chou JL,  Huang HB , Tseng CW, Wu SF, Chan MWY (2013) SOCS-1 Promoter Methylation and Treatment Response in Chronic Hepatitis C Patients Receiving Pegylated-Interferon/Ribavirin.  J. Clin. Immunol.   33, 1110-1116.
  30. Yu HC, Lu MC, Li C, Huang HL, Huang KY, Liu SQ, Lai NS*, and  Huang HB* (2013) Targeted delivery of an antigenic peptide to the endoplasmic reticulum: application for development of a peptide therapy for ankylosing spondylitis.  PLoS One   8(10), e77451
  31. Chen YR,  Huang HB, Lo CJ, Wang CC, Ho LK, Liu HT, Shiao MS, Lin TH*, Chen YC* (2013) Effect of Alanine Replacement of L17 and F19 on the aggregation and neurotoxicity of arctic-type Aβ40.  PLoS One   8(4), e61847.
  32. Lee MC, Lu MC, Lai NS, Liu SC, Yu HC, Lin TY, Hung SP,  Huang HB, Yin WY (2013) Renal dysfunction by BK virus infection is correlated with activated T cell level in renal transplantation.  J. Surg. Res.   180, 330-336.
  33. Liu MC, Lai NS, Yin WY, Yu HC,  Huang HB, Tung CH, Huang KY, Yu CL (2013). Anti-citrullinated protein antibodies activated ERK1/2 and JNK mitogen-activated protein kinases via binding to surface-expressed citrullinated GRP78 on mononuclear cells.  J. Clin. Immunol.   33, 558-566.
  34. Ho TY, Huang KY, Huang TT, Huang YS, Ho HC, Chou P, Lin CH, Wei CK, Lian WC, Chen TC,  Huang HB, Lee CC. (2012) The impact of influenza vaccinations on the adverse effects and hospitalization rate in the elderly: a national based study in an asian country.  PloS One  7(11), e50337.
  35. Liu MC, Lai NS, Chen HC, Yu HC, Huang KY, Tung CH.  Huang HB, Yu CL. (2012) Decreased microRNA(miR)-145 and increased miR-224 expression in T cells from patients with systemic lupus erythematosus involved in lupus immunopathogenesis.  Clin. Exp. Immunol.   171, 91-99.
  36. Lai NS, Yu CL, Yin WY, Yu HC,  Huang HB, Tung CH, Lu MC. (2012) Combination of nifedipine and subtherapeutic dose of cyclosporin additivity suppresses mononuclear cells activation of patients with rheumatoid arthritis and normal individuals via Ca2+-calcineurin-nuclear factor of activated T cells pathway.  Clin. Exp. Immunol.   168, 78-86.
  37. Wang TF, Lai NS, Huang KY, Huang HL, Lu MC, Lin YS, Chen CY, Liu SQ, Lin TH*,  Huang HB*(2012) Identification and characterization of the actin-binding motif of phostensin.   Int. J. Mol. Sci.   13, 15967-15982.
  38. Wang CC,   Huang HB, Tsay HJ, Shiao MS, WinstonWu WJ, Chen YC*, Lin TH* (2012) Characterization of Aβ aggregation mechanism probed by congo red.   J. Biomol. Struct. Dyn.   30, 160-169.
  39. Chuang TT, Ong PL, Wang TF,  Huang HB, Chi MC, Lin LL (2012) Molecular characterization of a novel trehalose-6-phosphate hydrolase, TreA, from Bacillus licheniformis.  Int. J. Biol. Macromol.  50, 459-470.
  40. Lin YS1, Huang KY1, Wang TF, Huang HL, Yu HC, Yen JY, Hung SH, Liu SQ, Lai NS*,  Huang HB* (2011) Immunolocalization of Phostensin in Lymphatic Cells and Tissues.  J. Histochem. Cytochem.   59, 741-749.
  41. Chen YC, Lee CC, Huang CY,   Huang HB, Yu CC, Ho YC, Su YC (2011) Haptoglobin polymorphism as a risk factor for chronic kidney disease: A case-control study.  Am. J. Nephrol.  33, 510-514.
  42. Chen YR1,   Huang HB1, Lo CJ, Wang CC, Su CL, Liu HZ, Shiao MS, Lin TH*, Chen YC* (2011) Aβ40(L17A/F19A) mutant diminishes the aggregation and neurotoxicity of Aβ40. Biochem.   Biophys. Res. Commum.   405, 91-95.
  43. Lu MC, Lai NS, Yu HC,   Huang HB, Hsieh SC, Yu CL (2010) Anti-citrullinated protein antibodies bind surface-expressed citrullinated Grp78 on monocytes/macrophages and stimulate tumor necrosis factor α production.  Arthritis Rheum.  62, 1213-1223.
  44. Chen CY, Lai NS, Yang JJ, Huang HL, Hung WC, Li C, Lin TH*,   Huang HB* (2010) FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1).   Biochem. Biophys. Res. Commum.  391, 698-702.
  45. Huang YC, Chen YC, Tsay HJ, Chyan CL, Chen CY,   Huang HB* and Lin TH* (2010) The effect of PKA-Phosphorylation on the structure of inhibitor-1 studied by NMR spectroscopy.   J. Biochem.   147, 273-278.
  46. Lai NS, Wang TF, Wang SL, Chen CY, Yen JY, Huang HL, Li C, Huang KY, Liu SQ, Lin TH*,   Huang HB* (2009) Phostensin caps to the pointed end of actin filaments and modulates actin dynamics.  Biochem. Biophys. Res. Commum.   387, 676-681.
  47. Chang CH, Peng CH, Chen KC,   Huang HB, Chiu WT, Peng RY (2009) Shock membrane electropotential drops and limited diffusive distance of β-amyloids in cerebral neurons are detrimental enhancement to Alzheimer’s diseases.  Colloids and Surfaces B: Biointerfaces.  73, 339-345.
  48. Chi MC, Ong PL, Hsu WE, Chen YH,   Huang HB*, Lin LL* (2008) Role of the invariant Asn345 and Asn435 residues in a leucine aminopeptidase from Bacillus kaustophilus as evaluated by site-directed mutagenesis.   Int. J. Biol. Macromol.  43, 481-487.
  49. Chi MC, Lu RQ, Lin LL*,   Huang HB* (2008) Characterization of Bacillus kaustophilus leucine aminopeptidase immobilized in Ca-alginate/k-carrageenan beads.   Biochem. Eng. J.   39, 376-382.
  50. Chi MC, Liu JS, Wang WC, Lin LL*,   Huang HB* (2007) Site-directed mutagenesis of the conserved Ala348 and Gly350 residues at the putative site of Bacillus kaustophilus leucine aminopeptidase.   Biochimie   90, 811-819.
  51. Kao SC, Chen CY, Wang SL, Yang JJ, Hung WC, Chen YC, Lai NS, Liu HZ, Huang HL, Chen HC, Lin TH*,   Huang HB* (2007) Identification of phostensin, a PP1 F-actin cytoskeleton targeting subunit.   Biochem. Biophys. Res. Commum.   356, 594-598.
  52. Huang HB*, Chen YC, Lee TT, Huang YC, Liu HT, Liu CK, Tsay HJ, Lin TH* (2007) Structural and biochemical characterization of inhibitor-1a.   Proteins   68, 779-788.
  53. Liao MQ, Tzeng YI, Chang LY,   Huang HB, Lin TH, Chyan CL, Chen YC (2007) The correlation between neurotoxicity, aggregative ability and secondary structure studies by sequence truncated Aβ peptides.   FEBS Let.   581, 1161-1165.
  54. Chang LS, Chen KC, Lin SR,   Huang HB (2006) Purification and characterization of Ophiophagus Hannah cytotoxin-like proteins.   Toxicon   48, 429-436.
  55. Chen YR,   Huang HB, Chyan CL, Shiao MS, Lin TH, Chen YC (2006) The effect of Aβ conformation on the metal affinity and aggregation mechanism studies by circular dichroism spectroscopy.   J. Biochem.   139, 733-740.
  56. Chang LS, Lin SR,   Huang HB (2006) Disulfide isomerization and thiol-disulfide exchange of long neurotoxins from the venom of   Ophiophagus hannah.  Arch. Biochem.   Biophy. 454, 181-188.
  57. Chen YM, Lee TH, Lee SJ,   Huang HB, Huang R, Chou HN (2006) Comparison of protein phosphatase inhibition activies and mouse toxicities of microcystins.   Toxicon   47, 742-746.
  58. Chi MC,   Huang HB, Liu JS, Wang WC, Liang WC, Lin LL (2006) Residues threonine 346 and leucine 352 are critical for the proper function of Bacillus kaustophilus leucine aminopeptidase.   FEMS Microbiol.   Lett. 260, 156-161.
  59. Lin TH, Tsai PC, Liu HT, Chen YC, Wang LS, Shie FK,   Huang HB* (2005) Characterization of the protein phosphatase 1-binding motifs of inhibitor-2 and DARPP-32 by surface plasmon resonance.   J. Biochem.   138, 697 – 700.
  60. Chyan CL, Huang PC, Lin TH, Huang JW, Lin SS,   Huang HB, Chen YC (2005) Purification, crystallization and preliminary crystallographic studies of a calmodulin-OLFp hybrid molecule.   Acat. Cryst.   F61, 785-787.
  61. Huang HB, ChiMC, Hsu WH, Liang WC, Lin LL (2005) Construction and one-step purification of Bacillus kaustophilus leucine aminopeptidase fused to the starch-binding domain of Bacillus sp. Strain TS-23 α-amylase. Biopro.   Biosyst. Eng.   27, 389-398.
  62. Lin TH,   Huang HB, Wei HA, Shiao SH, Chen YC (2005) The effect of temperature and lipid on the conformational transition of gramicidin A in lipid vesicles.   Biopolymers   78, 179-186.
  63. Lin, T.H., Huang, Y.C., Chin, M.L., Chen, Y.C., Jeng, H.H., Lin, F.M., Shiao, M.S., Horiuchi, A., Greengard, P., Nairn, A.C., and   Huang, H.B.* (2004) Backbone 1H, 15N, and 13C resonance assignments of DARPP-32 (dopamine and cAMP-regulated phosphoprotein, Mr. 32,000)—a protein inhibitor of protein phosphatase-1   J. Biomol. NMR.   28, 413-414.
  64. Lin TH, Huang JW,   Huang HB, Chen YC, Liu CY, Lo CJ, Tang TC. Chyan CL (2004) 1H, 15N, and 13C resonance assignments of complexed with the calmodulin-binding domain of protein phosphatase 2B.   J. Biomol. NMR.   29, 531-532.
  65. Lin, T.H., Chen, Y.C., Chyan, C.L., Tsay, L.H., Tang, T.C., Jeng, H.H., Lin, F.M., and   Huang, H.B.* (2003) Phosphorylation of glycogen synthase kinase of inhibitor-2 does not change its structure in free state.   FEBS Lett.   554, 253-256.
  66. Watanabe, T., da Cruz e Silva, E.,   Huang, H.B., Starkova, N., Kwon, Y.G., Horiuchi, A., Greengard, P., and Nairn, A.C. (2003) Preparation and characterization of recombinant protein phosphatase 1.   Methods Enzymol.   366, 319-338.
  67. Sung, Y.J., Cheng, C.I., Chen, C.S.,   Huang, H.B., Wu, P.C., Shiao, M.S., and Tsay, H.J. (2003) Distinct mechanisms account for β-amyloid toxicity in PC12 and Differentiated PC12 neuronal cells.   J. Biomed. Sci.   10, 379-388.
  68. Colby, D.A., Liu, W., Sheppeck, J.E.,   Huang, H.B., Nairn, A.C., and Chamberlin, A.R. (2003) A new model of tautomycin-PP1 complex that is not analogous to the corresponding okadaic acid structure. Bioorg.   Med. Chem. Lett.   13, 1601-1605.
  69. Liu, W., Colby, D.A., Sheppeck, J.E.,   Huang, H.B., Nairn, A.C., and Chamberlin, A.R. (2003) The selective inhibition of phosphatase by natural toxin: the anhydride domain of tautomycin is not a primary factor in controlling PP1/PP2A Sselectivity. Bioorg.   Med. Chem. Lett.   13, 1597-1600.
  70. Chang, L.S., Liou, J.C., Lin, S.R., and   Huang, H.B. (2002) Purification and characterization of neurotoxin from the venom of Ophiophagus hannah (king cobra).   Biochem. Biophys. Res. Commun.   294, 574-578.
  71. Liu, H. T., Lin, T. H., Kuo, H. C., Chen, Y. C., Tsay, H. J., Jeng, H. H., Tsai, P. C., Shie, F. C., Chen, J. H., and   Huang, H. B. * (2002) Identification of the alternative splice products encoded by the human protein phosphatase inhibitor-1 gene.   Biochem. Biophys. Res. Commun.   291, 1293-1296.
  72. Hung, C.J., Huang, P.C., Lu, S.C., Li, Y.H.,   Huang, H.B., Lin, B.F., Chang, S.J., and Chou, H.F. (2002) Plasma homocysteine levels in Taiwanese vegetarians are higher than those of omnivores.   J. Nutr.  132, 152-158.
  73. Gulledge, B., Aggen, J.B.,   Huang, H.-B., Nairn, A.C. and Chamberlin, R. (2002) The microcystins and nodularins: cyclic polypeptide inhibitors= of PP1 and PP2A.   Current Medicinal Chemistry   9, 1903-1915.
  74. Chyan, C.L., Tang, T.C., Chen, Y.C., Liu, H.T., Lin, F.M., Liu, C.K., Hsieh, M.J., Shiao, M.S.,   Huang, H.B., and Lin, T.H*. (2001) Backbone 1H, 15N, and 13C resonance assignments of inhibitor-1—a protein inhibitor of protein phosphatase-1.   J. Biomol. NMR   21, 287-288.
  75. Chang, L.S., Chung, C.,   Huang, H.B. , Lin, S.R. (2001) Purification and characterization of a chymotrypsin inhibitor from the venom of Ophiophagus hannah (king cobra).   Biochem. Biophys. Res. Commun.   283, 862-867.
  76. Huang, H.B., Chen, Y.C., Horiuchi, A., Tsai, L.H., Liu, H.T., Chyan, C.L., Lin, F.M., Greengard, P., Nairn, A.C., Hsieh, M.J., Liu, C.K., Shiao, M.S., and Lin, T.H. (2001) Backbone 1H, 15N, and 13C resonance assignments of ARPP-19.   J. Biomol.NMR   19, 383-384.
  77. Watanabe, T.,   Huang, H.B., Horiuchi, A., Hsieh-Wilson, L., Allen, P.B., Shenolikar, S., Greengard, P. and Nairn, A.C. (2001) Protein phosphatase 1 regulation by inhibitors and targeting subunits.   Proc. Natl. Acad. Sci.   98, 3080-3085.
  78. Huang, H. B. , Chen, Yi-chen, Tsai, Li-huang, Wang, Hui-chun, Lin, Fang-min, Shiao, Ming-shi, and Lin, Ta-hsien Lin (2000) Expression and assignment of the 1H, 15N, and 13C resonances of inhibitor-2, the inhibitor of protein phosphatase-1.   J. Biomol. NMR.  17, 359-360.
  79. Chang, L. S., Lin, S. R., Hu, W. P., Wu, B. N., and   Huang, H. B. (2000) Structure-function studies on Taiwan cobra long neurotoxin homolog.   Biochem. Biophys.Acta   1480, 293-301.
  80. Connor, J.H., Frederick, D.,   Huang, H.B. , Yang, J., Helps, N.R., DePaoli-Roach, A., Cohen, P.T.W., Tatchell, K., Nairn, A.C. and Shenolikar, S. (2000) Cellular Mechanism Regulating Protein Phosphatase-1: A key Functional Interaction between Inhibitor-2 and the PP1 Catalytic Subunit.   J. Biol. Chem  . 275, 18670-18675.
  81. Chang, L.S., Huang, H.B., and Lin, S.R. (2000) The multiplicity of cardiotoxins from Naja naja atra (Taiwan cobra) venom. Toxicon   38, 1065-1076.
  82. Chang,L.S., Lin, S.K.,   Huang, H.B. and Hsiao, H.S.M. (1999) Genetic organization of alpha-bungarotoxin from Bungarus multicinctus (Taiwan banded krait): Evidence showing the production of alpha-bungarotoxin isotoxins is not derived from edited mRNA.   Nucleic Acids Research   27, 3970-3975.
  83. Hunag, H.B. *, Horiuchi, A., Watanabe, T., Shih, S.R., Tsay, H.J., Li., H.C., Greengard, P. and Nairn, A.C*. (1999) Characterization of the inhibition of protein phosphatase-1 by DARPP-32 and inhibitor-2.   J. Biol. Chem   274 , 7870-7878.
  84. Aggen, J.B., Humphrey, J.M., Gauss, C.M.,   Huang, H.B. , Nairn, A.C. and Chamberlin, R. (1999) The design, synthesis, and biolobical evaluation of analogs of the serine-threonine protein phosphatase 1 and 2A selective inhibitor microcystin LA: rational modifications imparting PP-1 selectivity.   Bioorg. Med. Chem   , 543-564.
  85. Lin S.R.,   Huang, H.B., Wu, B.N. and Chang, L.S. (1998) Characterization and cloning of long neurotoxin homolog from naja naja atra.   Biochem. Mol. Biol. Int.   46, 1211-1217.
  86. Kwon, Y.G.,   Huang, H.B. , Desdouits, F., Girault, J.A., Greengard, P. and Nairn, A.C. (1997) Characterization of the interaction between protein phosphatase-1 and DARPP-32: DARPP-32 peptide antagonize the interaction of protein phosphatase-1 with binding proteins.   Proc. Natl. Acad. Sci. USA   94, 3536-3541.
  87. Huang, H.B. , Horiuchi, A., Goldberg, J., Greengard, P. and Nairn, A.C. (1997) Site-directed mutagenesis of amino acid residues of protein phosphatase-1 involved in catalysis and inhibitor binding.   Proc. Natl. Acad. Sci. USA   94, 3530-3535.

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