Tel: (05)2720411 ext. Office 66502 Lab 61515

 Fax: (05)2722871

 Email: biomlh@ccu.edu.tw

• 1992 B.S. Biology, Tunghai University (東海大學生物系 學士)
• 1994 M.S. Botany, National Chung Hsing University (國立中興大學 植物學研究所 碩士)
• 2002 Ph.D. Neuroscience, National Yang Ming University(國立陽明大學神經科學 博士)

Associate Professor, Department of Biomedical Sciences, National Chung Cheng University

• 2011/8 - present Associate Professor, Department of Biomedical Sciences, National Chung Cheng University(國立中正大學生物醫學科學系 副教授)
• 2003/8- 2011/7 Assistant Professor, Department of Life Science, National Chung Cheng University.(國立中正大學生命科學系 助理教授)
• 2003/1-6 Distinguished Post-Doctoral Fellow, Institute of Molecular Biology, Academia Sinica, Taiwan.(中央研究院分子生物學研究所 卓越博士後研究員)

• Genetics(遺傳學)
• Neural development(神經發育學)

• 2000：Award of excellent Ph.D. thesis sponsored by Dr. Chien-Tien Hsu in CSCMB The 8th Symposium on Recent Advances in Cellular and Molecular Biology.(第八屆細胞及分子生物新知研討會，徐千田優秀論文獎第一名)
• 1999：Outstanding Poster Award in CSCMB The 7th Symposium on Recent Advances in Cellular and Molecular Biology.(第七屆細胞及分子生物新知研討會，優秀壁報獎)
• 1998：Poster Award in CSCMB The 6th Symposium on Recent Advances in Cellular and Molecular Biology. (第六屆細胞及分子生物新知研討會，優秀壁報獎佳作)

The story of Kuduk

The human transmembrane protein TMEM258 is a component of the evolutionarily conserved oligosaccharyltransferase (OST) complexes, which are essential for N-linked protein glycosylation. Aberrant TMEM258 expression is associated with spinocerebellar ataxia type 20 and inflammatory bowel diseases. We previously identified the Drosophila ortholog Kuduk (Kud) and found that Kud modulates the nuclear envelope architecture and autophagy. Interestingly, we found that Kud can localize not only at endoplasmic reticulum where OST-mediated glycosylation occurs, but also at the nuclear envelope and unidentified cytoplasmic puncta. The observation suggests that Kud exhibits OST-independent functions. Our study will provide the knowledge not only for the molecular functions of Kud, but also for the pathogenesis of the diseases associated with TMEM258.

人類跨膜蛋白TMEM258是演化上具有高度保留性之寡醣轉移酶（OST）複合體的次單元，OST複合體負責調控蛋白質的N-醣基化，TMEM258的表現異常與脊髓小腦失調症和炎症性腸病的發生有關，但其病理機制尚待釐清。我們先前的研究找到TMEM258在果蠅的同源蛋白Kuduk(Kud)，並證實Kud能夠調控細胞核的形態(圖A-B)及細胞自噬作用(圖C-E)。我們發現Kud除了座落在OST複合體作用的內質網，亦會座落於核膜和一些未知的細胞質囊泡上，這表示Kud可能具有獨立於OST複合體以外的功能。我們將深入探討Kud的分子功能，進而釐清TMEM258相關疾病之病理機轉。

Neural development

Dendrites are cell-specific characteristics of neurons, and are critical to receive stimulations from upstream neurons or the environment. Aberrant dendritic morphologies impair neuronal circuits and are correlated with neurological and neurodevelopmental disorders. The knowledge of mechanisms for controlling the dendrite pattering remains largely unclear. The Drosophila nervous system is well characterized in the cellular number and morphology. We aim to use Drosophila as a platform to carry out genetic screenings to study the cellular machineries for dendrite morphogenesis.

樹突是神經元特有的構造，負責接收來自上游神經元或外在環境的刺激。樹突形態異常會損害神經元迴路並可能導致神經發育相關之疾病。目前對於調控樹突形態發育之機制仍有許多尚未明瞭之處。果蠅是一個歷史悠久的模式生物，具有完備的遺傳資料，其神經系統的細胞數目及形態已被研究得很清楚。本實驗室利用果蠅進行遺傳篩選，尋找與探討調控神經樹突形態形成之細胞分子機制。

• 國科會 92-2321-B-194-002- unzipped在果蠅神經發育中之功能分析 (2003/12 ~ 2004/07)
• 國科會 93-2311-B-194-001- 決定果蠅周圍神經突生長點之機制的研究 (2004/08 ~ 2005/07)
• 國科會 94-2311-B-194-004- Wnt訊息路徑之新調節分子的研究 (2005/08 ~ 2006/07)
• 國科會 95-2311-B-194-001- MY3 Molecular mechanism of cell polarity and morphogenesis in Drosophila (2006/08 ~ 2009/07)
• 國科會 98-2311-B-194-002- 過度活化之TOR訊息造成果蠅眼睛受損的機制 (2009/08 ~ 2010/07)
• 國科會99-2311-B-194-001-MY3果蠅眼睛發育中TOR對Jak/STAT訊息路徑的調控機制 (2010/08~2013/07)
• 國科會 102-2311-B-194 -002 - 一個新核膜蛋白的生物功能與機制 (2013/08~2014/07)
• 科技部 103-2311-B-194 -001 -MY3 一個新的、座落在核膜及高基氏體之蛋白質的鑑識與功能分析 (2014/08~2017/07)
• 科技部 103-2745-B-001 -001 -ASP 神經元樹突內的細胞作用機制(1/5) (2014/08~2015/07)
• 科技部 104-2745-B-001 -001 -ASP 神經元樹突內的細胞作用機制(2/5) (2015/08~2016/07)
• 科技部 105-2745-B-001 -001 -ASP 神經元樹突內的細胞作用機制(3/5) (2016/08~2017/07)
• 科技部 107-2745-B-001 -001 -ASP 神經元樹突內的細胞作用機制(5/5) (2018/08~2019/07)
• 科技部 107-2311-B-194 -001 - 細胞自噬作用內之新跨膜蛋白Kuduk/TMEM258的分子機制 (2018/08~2019/10)
• 科技部 108-2311-B-194 -001 - 寡醣轉移酶複合體的次單元Kud/TMEM258調控細胞自噬作用之機制 (2019/08~2020/10)

1. Wang YH, Ding ZY, Cheng YJ, Chien CT, and Huang ML. An Efficient Screen for Cell-Intrinsic Factors Identifies the Chaperonin CCT and Multiple Conserved Mechanisms as Mediating Dendrite Morphogenesis. Frontiers in cellular neuroscience, 14, 311. (2020)
2. Ding ZY, Wang YH, Huang YC, Lee MC, Tseng MJ, Chi YH, and  Huang ML. Outer nuclear membrane protein Kuduk modulates the LINC complex and nuclear envelope architecture.  J Cell Biol  216, 2827-2841 (2017)
3. Lin MD, Lee HT, Wang SC, Li HR, Hsien HL, Cheng KW, Chang YD, Huang ML, Yu JK, Chen YH. Expression of phosphatase of regenerating liver family genes during embryogenesis: an evolutionary developmental analysis among Drosophila, amphioxus, and zebrafish. BMC Developmental Biology; 13:18 (2013)
4. Wang CC, Lo JC, Chien CT, Huang ML. Spatially controlled expression of the Drosophila pseudouridine synthase RluA-1.  International Journal of Developmental Biology;  55(2):223-7 (2011)
5. Ding ZY, Wang YH, Luo ZK, Lee HF, Hwang J, Chien CT, Huang ML. Glial cell adhesive molecule Unzipped mediates axon guidance in Drosophila.  Developmental Dynamic;  240(1):122-34. (2011)
6. Lu KT, Sun CL, Wo PY, Yen HH, Tang TH, Ng MC, Huang ML,  Yang YL. Hippocampal Neurogenesis following Traumatic Brain Injury was Mediated by Vascular Endothelial Growth Factor Receptor 2 and Raf/MEK/ERK Cascade.  Journal of Neurotrauma;  28(3):441-50. (2011)
7. Wang LC, Chen KY, Pan H, Wu CC, Chen PH, Liao YT, Li C, Huang ML,  Hsiao KM. Muscleblind participates in RNA toxicity of expanded CAG and CUG repeats in Caenorhabditis elegans.  Cellular and Molecular Life Sciences;  68(7):1255-67. (2011)
8. Wang YH, Huang ML. Organogenesis and tumorigenesis: Insight from the JAK/STAT pathway in the Drosophila eye.  Developmental Dynamic;  239(10):2522-33. (2010)
9. Wang YH, Huang ML. Reduction of Lobe leads to TORC1 hypoactivation that induces ectopic Jak/STAT signaling to impair Drosophila eye development.  Mechanisms of Development;  126(10):781-90. (2009)
10. Huang ML,  Hsu CH, Chien CT. The proneural gene amos promotes multiple dendritic neuron formation in the Drosophila peripheral nervous system.  Neuron   25(1): 57-67. (2000)