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De-Ching Chang


Contact information

 Tel: (05)2720411 ext. Office 66500 Lab 61506~61507

 Fax: (05)2722871

 Email: biodcc@ccu.edu.tw

Academic qualification 

Ph.D. in Virology and Oncology, Division of Biology, Kansas State University, USA, 1993.

Working Experience 
  • Aug. 2000-Present: Professor. Institute of Molecular Biology, National Chung Cheng University, Taiwan, ROC. Jun.
  • 2006-Aug. 2009: Dean. College of Science, National Chung Cheng University, Taiwan, ROC. Jun.
  • 2001-2008: Director. Biomedical Technology and Engineering Center, National Chung Cheng University, Taiwan, ROC. Aug.
  • 2000-2003: Director. Institute of Molecular Biology, National Chung Cheng University, Taiwan, ROC.
  • Aug. 1996-Jul. 2000: Professor / Chairman. Department of Microbiology and Immunology, Chung Shan Medical University. Taiwan, ROC.
  • Aug. 1997-Jul. 1998: Director. Library of Chung Shan Medical University. Taiwan, ROC.
  • Aug. 1995-Jul. 1996: Associate Professor / Chairman. Department of Microbiology and Immunology, Chung Shan Medical University. Taiwan, ROC.
  • Aug. 1993- Jul. 1996: Associate Professor. Department of Microbiology and Immunology, Chung Shan Medical University. Taiwan, ROC.
  • Jan. 1993-Jul. 1993: Postdoctoral Fellow, Section of Virology and Oncology, Division of Biology, Kansas State University. USA
  1. Shen, C.-H, M-C. Lin, C.-Y. Fang, P.-L. Chen, C.-C. Chou, C.-L. Tung, C.-N. Chao,  D.C. Chang* and M. Wang. (Jul. 2021) Suppression of bone metastatic castration-resistant prostate cancer cell growth by a suicide gene delivered by JC polyomavirus-like particles.  Gene Therapy.   doi.org/10.1038/s41434-021-00280-8.
  2. Lai, W.-H, C.-Y. Fang, M.-C. Chou, M.-C Lin, C.-H. Shen, C.-N. Chao, Y.-C. Jou,  D.C. Chang*, M. Wang. (Jun. 2021). Peptide-guided JC polyomavirus-like particles specifically target bladder cancer cells for gene therapy.  Scientific Reports. 2021 Jun 4;11(1):11889.
  3. Chen, S.-H., J.-M. Sun, B.-M. Chen, S.-C. Lin, H.-F. Chang, S. Collins,  D.C. Chang, S.-F. Wu, Y.-C. Lu, W. Wang, T. C. Chen, N. Kasahara, H.-E. Wang, and C.-K. Tai. (May 2020). Efficient Prodrug Activator Gene Therapy by Retroviral Replicating Vectors Prolongs Survival in an Immune-Competent Intracerebral Glioma Model.  International Journal of Molecular Sciences. 2020, 21, 1433;   doi:10.3390/ijms21041433.
  4. Lin, Y.-S., Y.-C. Shen, C.-Y. Wu, Y.-Y. Tsai, Y.-H. Yang, Y.-Y. Lin, F.-C. Kuan, C.-N. Lu, G.-H. Chang, M.-S. Tsai, C.-M. Hsu, R.-A. Yeh, P.-R. Yang, I-Y. Lee, L.-H. Shu, Y.-C. Cheng, H.-T. Liu, Y.-H. Wu, Y.-H. Wu and  D.C. Chang*. (Oct. 2019). Danshen improves survival of patients with breast cancer and dihydroisotanshinone I induces ferroptosis and apoptosis of breast cancer cells.  Frontiers in Pharmacology. 10:1226.   doi: 10.3389/fphar.2019.01226.
  5. Lin, M.-C., C.-H. Shen,  D.C. Chang and M. Wang (2019, Nov). Inhibition of human lung adenocarcinoma growth and metastasis by JC polyomavirus-like particles packaged with an SP-B promoter-driven CD59-specific shRNA.  Clinical Science  , 133(21):2159-2169. (SCI).
  6. Lin, M.-C, M. Wang, M.-C. Chou, C.-N. Chao, C.-Y. Fang, P.-L. Chen,  D.C. Chang , C.-H. Shen (2019, Jul). Gene therapy for castration-resistant prostate cancer cells using JC polyomavirus-like particles packaged with a PSA promoter driven-suicide gene.  Cancer gene therapy  , 26(7-8):208-215. (SCI).
  7. Lin Y.-S. Lin, Y.-Y. Lin, Y.-H. Yang, C.-L. Lin, F.-C. Kuan, C.-N. Lu, G.-H. Chang, M.-S. Tsai, C.-M. Hsu, R.-A. Yeh, P.-R. Yang, I-Y. Lee, L.-H. Shu, Y.-C. Cheng, H.-T. Liu, K.-D. Lee,  D.C. Chang and C.-Y. Wu (2018, May). Antrodia cinnamomea extract inhibits the proliferation of tamoxifen-resistant breast cancer cells through apoptosis and skp2/microRNAs pathway.  BMC Complementary and Alternative Medicine  , 18(1):152. (SCI).
  8. Chao C.-N., Y.-H. Yang, M.-S. Wu, M.-C. Chou, C.-Y. Fang, M.-C. Lin, C.-K. Tai, C.-H. Shen, P.-L. Chen,  D.C. Chang and M. Wang (2018, Feb). Gene therapy for human glioblastoma using neurotropic JC virus-like particles as a gene delivery vector. Scientific Reports  , 8(1): 2213. (SCI).
  9. Chen, S.-Y., R.-H. Teng, M. Wang, P.-L. Chen, M.-C. Lin, C.-H. Shen, C.-N. Chao, M.-K Chiang, C.-Y Fang, and  D.C. Chang. (2017, Jul).Rhodiolae kirliowii radixet rhizoma and crataegus pinnatifida fructus extracts effectively inhibit BK virus and JC virus infection of host cells.  Evidence-Based Complementary and Alternative Medicine  . 2017, ID 5620867. (SCI).
  10. Chao, C.-N., M.-C. Lin, C.-Y. Fang, P.-L. Chen,  D.C. Chang, C.-H. Shen, M. Wang (2016, Jun). Gene therapy for human lung adenocarcinoma using a suicide gene driven by a lung-specific promoter delivered by JC virus-like particles.  Plos one  , 11(6), e0157865. (SCI).
  11. Yeh, C-M, P-C Chen, H-Y Hsieh, Y-C Jou, C-T Lin, M-H Tsai, W-Y Huang, Y-T Wang, R-I Lin, S-S Chen, C-L Tung, S-F Wu, D.C. Chang, C-H Shen, C-D Hsu and M W.Y. Chan. 2015. Methylomics analysis identifies ZNF671 as an epigenetically repressed novel tumor suppressor and a potential non-invasive biomarker for the detection of urothelial carcinoma. Oncotarget , 6(30), 29555-72.
  12. Fang, C.-Y., Y.-D. Tsai, M.-C. Lin, M. Wang, P.-L. Chen, C.-N. Chao, Y.-L. Huang,  D.C. Chang and C.-H. Shen (2015,Jun). Inhibition of human bladder cancer growth by a suicide gene delivered by JC polyomavirus virus-like particles in a mouse model.  Journal of Urology  , 193(6), 2100-2106. (SCI).
  13. Fang, C.-Y., C.-H. Shen, M. Wang, P.-L. Chen, M. Chan, P.-H. Hsu and  D.C. Chang (2015, Sep). Global profiling of histone modifications in the polyomavirus BK virion minichromosome.  Virology  , 483, 1-12. (SCI).
  14. Chao, C.-N., Y.-L. Huang, M.-C. Lin, C.-Y. Fang, C.-H. Shen, P.-L. Chen, M. Wang,  D.C. Chang and C.-E. Tseng (2015, Jan). Inhibition of human diffuse large B-cell lymphoma growth by JC polyomavirus-like particles delivering a suicide gene.  Journal of Translational Medicine  , 27;13(1):29-38 (SCI)
  15. Tseng, C.-E., C.-M. Yeh, C.-Y. Fang, J. Shay, P.-L. Chen, M.-C. Lin, D.C. Chang and M. Wang. 2014. Detection of human JCPyV and BKPyV in diffuse large B-cell lymphoma of the GI tract. European Journal of Clinical Microbiology and Infectious Diseases. 33:665-672.
  16. Lin, M.-C., M. Wang, C.-Y. Fang, P.-L. Chen, C.-H. Shen, D.C. Chang. 2014. Inhibition of BK virus replication in human kidney cells by BK virus large tumor antigen-specific shRNA delivered by JC virus-like particles. Antiviral Research. 103:25–31.
  17. Tsay, G.J.,Y.-F. Hsieh, M. Wang, D.C. Chang, J.T. Chang and M. Zouali. 2013. Targeting the IL-10 pathway by RNA interference has beneficial effects o the development of experimental lupus. European Journal of Inflammation. 11:43-54
  18. Liaw, Y.-C., C.-H. Chen, K.-H. Shu, C.-Y. Fang, W.-C. Ou, P.-L. Chen, Shen, M.-C. Lin, D.C. Chang, M. Wang. 2012. Recombined sequences between the non-coding control regions of JC and BK viruses found in the urine of a renal transplantation patient.Virus Genes. 45:581-584.
  19. Fang, C.-Y., P.-Y. Lin, W.-C. Ou, P.-L. Chen,.C.-H. Shen, D.C. Chang, M. Wang. 2012. Analysis of the size of DNA packaged by the human JC virus-like particle. Journal of Virological Methods.  182:87– 92.
  20. Chang, C.-F., M. Wang, W.-C. Ou, P.-L. Chen, C.-H. Shen, P.-Y. Lin, C.-Y. Fang, D.C. Chang. 2011. Human JC virus-like particles as a gene delivery vector. Expert Opinion on Biological Therapy. 11:1169-1175.
  21. Chen, P.-C. M.-H. Tsai, S. K.H. Yip, Y.-C. Jou, C.-F. Ng, Y. Chen, X. Wang, W. Huang, C.-L. Tung, G.C.-W. Chen, M. M.-S. Huang, J.H.M. Tong, E.-J. Song, D.C. Chang, C.-D. Hsu, K.-F. To, C.-H. Shen and M.W.Y. Chan. 2011. Distinct DNA methylation epigenotypes in bladder cancer from different Chinese sub-populations and its implication in cancer detection using voided urine. BMC Medical Genomics. 4:45.
  22. Shen, C.-H, J.-D. Wu, C.-D. Shu, Y.-C. Jou, C.-T. Lin, M. Wang, S.-F. Wu, M.W.Y. Chan, M.-K. Chiang, C.-Y. Fang, and D.C. Chang. 2011. The high incidence of JC virus infection in urothelial carcinoma tissue in Taiwan. Journal of Medical Virology. 83: 2191–2199.
  23. Chen, P.-L., P.-H. Hsu, C.-Y. Fang, C.-F. Chang, W.-C. Ou, M. Wang and D.C. Chang. 2011. Phosphorylation of Ser-80 of VP1 and Ser-254 of VP2 are essential for human BK virus propagation in tissue culture.Journal of General Virology. 92:2637-2645.
  24. C.-Y. Fang, M. Wang, C.-Y. Fang, P.-L. Chen, S.-F. Wu, M.-W.Y. Chan and D.C. Chang. 2011. Analysis of DNA methylation in human BK virus. Virus Genes. 43, 201-207.
  25. Chen, C.-H., M.-C. Wen, M. Wang, J.-D. Lian, C.-H. Cheng, M.-J. Wu, T.-M. Yu, Y.-W. Chuang, D.C. Changand K.-H. Shu. 2010. High Incidence of Malignancy in Polyomavirus-Associated Nephropathy in Renal Transplant Recipients. Transplantation Proceedings. 42:817–818.
  26. Chou, M.-I., M. Wang, J.-T. D.C. Chang Chang , Y-F. Hsieh, M. Zoua and G. J. Tsay. 2010.Efficient transfection of IL-10 RNAi by JC virus as a gene delivery vector in both murine macrophage cells and BALB/c mice. The Journal of Biomedical Science. 17:51-59.
  27. Shen, C.-H., H.-Y. Hsieh, Y.-H. Wang, S.-Y. Chen, C.-L. Tung, J.-D. Wu, C.-T. Lin, C.-D. Hsu and D.C. Chang. 2010. High DKK1 expression in patients with urothelial carcinoma.Experimental and Therapeutic Medicine. 1:893-898.
  28. Fang, C.-Y., H.-Y. Chen, M. Wang, P.-L. Chen, C.-F. Chang, L.-S. Chen, C.-H. Shen, W.-C. Ou, M.-D. Tsai, P.-H. Hsu, and D.C. Chang. 2010. Global Analysis of Modifications of the Human BK Virus Structural Proteins by LC-MS/MS. Virology. 402:164-176.
  29. Chen, L.-S., M. Wang, W.-C. Ou, C.-Y. Fung, P.-L. Chen, C.-F. Chang, W.-S. Huang, J.-Y. Wang, P. Y. Lin and D.C. Chang. 2010. Efficient gene transfer using the human JC virus-like particle that inhibits human colon adenocarcinoma growth in a nude mouse model. Gene Therapy. 17:1033-1041.
  30. Chang, T.-L., M.-T. Yu, Y.-C. Ho, C.-H. Shen, D.-W. Liu, Chin Li, D.C. Chang, S.-F. Wu. 2010. Dysfunction of natural killer cells in patients with transitional cell carcinoma. Cancer Letter. 291:39-45.
  31. Lin P.-Y, C.-Y Fung, F.-P. Chang, W.-S. Huang, W.-C. Chen, J.-Y. Wang, D.C. Chang. 2008. Prevalence and genotype identification of human JC virus in colon cancers in Taiwan. Journal of Medical Virology.80:1828-1834.
  32. Chen, M.-H, S.-H. Chen, Q.-F., Wang, J.-C., Chen, D.C. Chang, S.-L. Hsu, Y.-W. Liu. 2008. The molecular mechanism of gypenosides-induced G1 growth arrest of rat hepatic stellate cells. J. Ethnopharmacol.117:309-317.
  33. Wen, M.-C., J.-D. Lian, H.-R. Chang, K.-H. Su, M.-J. Wu, C.-H. Chen, Y.-J. Jan, J. Wang, D.C. Chang. 2007. Polyomavirus Nephropathy in renal allograft: prevalence and correlation of histology and graft failure. Nephrology. 12:615-619.
  34. Chang, Y.-Y., M.-.Y. Lan, C.-H. Peng, H.-S. Wu, D.C. Chang, J.-S. Liu. 2007. Progressive multifocal leukoencephalopathy in an lmmunocompetent Taiwanese patient. J. Formos. Med. Assoc. 106:60-64.
  35. Chen M.-H., J.-C. Chen, C.-C. Tsai, W.-C. Wang, D.C. Chang, D.-G.Tu, H.-Y. Hsieh. 2005. The role of TGF-beta 1 and cytokines in the modulation of liver fibrosis by Sho-saiko-to in rat's bile duct ligated model. J. Ethnopharmacol.97:7-13.
  36. Wang, M.-L., T.-S. Tzou, W.-C. Ou, C.-F. Chang, C.-Y. Fung, L.-S. Chen and D.C. Chang. 2004. Inhibition of SV40 LT expression in human fetal glial cells by antisense oligodeoxynucleotide delivered by JCV virus-like-particle. Human Gene Therapy. 15:1077-1090.
  37. Chen, M.-H., J.-C. Chen, C.-C. Tsai, W.-C. Wang, D.C. Chang, C.-C. Lin and H.-Y. Hsieh. 2004. Herbal Medicine: Sho-saiko-to prevents liver fribrosis induced by bile duct ligation in rats. The American Journal of Chinese Medicine. 32:195-207.
  38. Wen, M.-C., C.-L. Wang, M.-L. Wang, C.-H. Cheng, M.-J. Wu, C.-H. Chen, K.-H. Shu and D.C. Chang. 2004. Association of JC virus with tubulointerstitial nephritis in a renal allograft recipient. Journal of Medical Virology. 72:675-678.
  39. D.C. Chang. 2004. Gene delivery by JC virus-like particle.Journal of NeuroVirology. Volume 10, Supplement 2, 26, DOI: 10.1080/13550280490469699
  40. Chen, M.-H., J.-C. Chen, D.C. Chang, W.-C. Wang and H.-Y. Hsieh. 2003. Effect of Xiao-Chai-Hu-Tang on anti-oxidation and suppression of the ito cell induced by bile duct ligation in rats. Journal of International Chinese and Western Medicine. 4:45-52.
  41. Huang, Y.-L., M.-L. Wang, W.-C. Ou, C.-Y. Fung, L.-S. Chen and D.C. Chang. 2003. Analysis of DNA binding activity of the JCV minor capsid protein VP2. Journal of NeuroVirology. 9:21-24.
  42. Chang, H., M.-L. Wang, R.-Tai Tsai, H.-S. Lin, J.-S. Huan, W.-C. Wang, and D.C. Chang. 2002. High incidence of JC viruria in JC seropositive older individuals. Journal of NeuroVirology. 8:1-5.
  43. Ou, W.-C., L.-H. Chen, M.-L. Wang, T.-H. Hseu and D.C. Chang. 2001. Analysis of minimal sequences on JC virus VP1 required for capsid assembly. Journal of NeuroVirology. 7:298-301.
  44. Chen, P.-L. M.-L. Wang, W.-C. OuC.-K. Lii, and D.C. Chang. 2001. Disulfide bonds stabilize JC virus capsid-like structure by protecting calcium ions from chelation. FEBS Letter. 500:109-113.
  45. Ou, W.-C., M. Wang, H. Chang, G. J. Tasy, T.-H. Hseu and D.C. Chang. 2001. Identification of a DNA encapsidation sequence for human polyomavirus pseudovirion formation. Journal of Medical Virology. 64:366-373.
  46. Chen, C.H., M.C. Wen, M.L. Wang, J.D. Lian, M.J. Wu, C.H. Cheng, K.H. Shu and D.C. Chang, 2001. A regulatory region rearranged BKV is associated with tubulointerstitial nephritis in a rejected kidney allograft. Journal of Medical Virology. 64:82-88.
  47. Wang, M.-L., R.-T. Tsai, W.-C. Ou, C.-K. Lin, G. J. Tsay, H. Chang and D.C. Chang. 2000. Treatment with cytotoxic immunosuppression agents increases urinary excretion of JCV in patients with autoimmune disease.” Journal of Medical Virology. 62:505-510.
  48. Wang, G.-Shern, C.-H. Chen, and D.C. Chang. 2000. Application of Molecular Biology in Clinical Medicine. Show-Chwan Medical Journal. 2:59-63.
  49. D.C. Chang 2000. Investigation of human polyomavirus. Life Sciences Newsletter, NSC. 14:3-5.
  50. D.C. Chang, C. Sugimoto, M.-L. Wang, R.-T. Tsai, and Y. Yogo. 1999. JC virus genotypes in the Taiwan aboriginal tribe (Bunun): implication for its population history. Archives of Virology. 144:1081-1090.
  51. Wang, M-L, T.-Y. Tzeng, C.-Y. Fung, W.-C. Ou, R.-T. Tsai, C.-H. Chen and D.C. Chang. 1999. Human anti-JC virus serum reacts with native but not denatured JC virus major capsid protein VP1. Journal of Virological Methods. 78:171-176.
  52. Ou, W.-C., M.-L. Wang, C-Y. Fung, R.-T. Tsai, P-C. Chao, T.-H. Hseu, and D.C. Chang . 1999. The major capsid protein VP1 of human JCV expressed in E. coli is able to self-assemble into a capsid-like particle and deliver exogenous DNA into human kidney cells. Journal of General Virology. 80:39-46.
  53. Tsai, R.-T., M.L. Wang, W.-C. Ou, Y.-L. Lee, S.-Y. Li, C.-Y. Fung, Y.-L. Huang, T.-Y. Tzeng, and D.C. Chang. 1997. Viruria incidence of JC virus is higher than that of BK virus in Taiwan. Journal of Medical Virology.52:253-257.
  54. Gillock, E.T., S. Rottinghaus, D.C. Chang, X. Cai, S.A. Smiley, and R.A. Consigli. 1997. Polyomavirus major capsid protein VP1 is capable of packaging cellular DNA when expressed in the baculovirus system. Journal of Virology. 71:2857-2865.
  55. D.C. Chang, C-Y. Fung, W-C. Ou, P-C. Chao, S-Y. Li, M-L Wang, Y-L. Huang, T-Y. Tzeng, and R.-T. Tsai. 1997. Self assembly of the JC virus major capsid protein VP1 expressed in insect cells. Journal of General Virology. 78:1435-1439.
  56. Ou, W.-C., R.-T. Tsai, M.-L. Wang, C-Y. Fung, T.-H. Hseu, and D.C. Chang . 1997. Genomic cloning and sequence analysis of Taiwan-3 human polyomavirus JC virus. Journal of The Formosan Medical Association. 96:511-516.
  57. D.C. Chang, W-C. Ou, K.-K. Liao, M-L Wang, and C-Y. Fung. 1996. "Cloning, Expressing and purification of the recombinant polyomavirus VP1 and characterization of its DNA binding domain. The Chung Shan Medical Journal. 7:1-12.
  58. D.C. Chang, Z-M., Liou, W-C. Ou, R.-T. Tsai, K-Z. Wang, M-L Wang, and C-Y. Fung. 1996. Production of the antigen and the antibody of the JC virus major capsid protein VP1. Journal of Virological Methods. 59:177-187.
  59. D.C. Chang, M-L Wang, W-C. Ou, R.-T. Tsai, C.-Y. Fung, and Y.-J. Hwang. 1996. A simple method to detect human polyomavirus DNA in urine by polymerase chain reaction. Journal of Virological Methods.58:131-136.
  60. D.C. Chang, R-T. Tsai, M-L Wang, and W-C. Ou. 1996. Different genotypes of human polyomaviruses found in patients with autoimmune diseases in Taiwan. Journal of Medical Virology. 48:204-209 .
  61. D.C. Chang, M-L Wang, W-C. Ou, M-S. Lee, H-N. Ho, and R-T. Tsai. 1996. Genotypes of human polyomaviruses in urine samples of pregnant individuals in Taiwan. Journal of Medical Virology. 48:95-101.
  62. Cai, X., D.C. Chang, and R.A. Consigli. 1994. Expression and purification of recombinant polyomavirus VP2 protein and its interactions with polyomavirus proteins. Journal of Virology. 68:7609-7613.
  63. Rodgers, R.E.D., D.C. Chang, X. Cai, and R.A. Consigli. 1994. Purification of Recombinant budgerigar fledgling disease virus VP1 capsid protein and its ability for in vitro Capsid Assembly. Journal. of Virology. 68:3386-3390.
  64. D.C. Chang, X. Cai, and R. A. Consigli. 1993. Characterization of the DNA binding properties of the polyomavirus structural proteins VP1, VP2, and VP3. Journal of Virology. 67:6327-6322.
  65. Haynes, J. I., D.C. Chang, and R.A. Consigli. 1993. Mutagenesis of the putative calcium-binding domain of the polyomavirus major capsid protein VP1. Journal of Virology. 67:2486-2495.
  66. D.C. Chang, A. Paulsen, T.C. Johnson, and R.A. Consigli. 1993. Virus protein assembly in microgravity. Advances of Space Research. 13:251-257.
  67. D.C. Chang, J.I. Haynes, J.H. Brady, and R.A. Consigli. 1993. Identification of an amino acid sequence in the polyomavirus capsid proteins that serve as nuclear localization signals. Transactions of the Kansas Academy of Science. 96:35-39.
  68. D.C. Chang, J.I. Haynes, J.N. Brady, and R.A. Consigli. 1992. Identification of a nuclear localization sequence in the polyomavirus structural protein VP2. Virology. 191:978-983.
  69. D.C. Chang, J.I. Haynes, J.H. Brady, and R.A. Consigli. 1992. The use additive and subtractive approaches to examine the nuclear localization sequence of the polyomavirus major capsid protein VP1. Virology.189:821-827.
  70. Consigli, R.A., J.H. Haynes, D.C. Chang, L. Grenz, and D. Richter. 1992. Early events in polyoma infection: adsorption, penetration, and nuclear transport. Transactions of the Kansas Academy of Science. 95:62-69.
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